Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16366 and 16367 are separated by 134 nucleotides16367 and 16368 are separated by 217 nucleotides16368 and 16369 are separated by 233 nucleotides16369 and 16370 are separated by 509 nucleotides mRNA_7998: 16366 - KOG3156 Uncharacterized membrane protein, at 176,627 to 177,709 16366 mRNA_7999: 16367 - K10994 RAD9A cell cycle checkpoint control protein RAD9A, at 177,844 to 179,868 16367 mRNA_8000: 16368 - K08991 MUS81 crossover junction endonuclease MUS81, at 180,086 to 183,760 16368 mRNA_8001: 16369 - K01092 E3.1.3.25, IMPA, suhB myo-inositol-1(or 4)-monophosphatase, at 183,994 to 185,593 16369 mRNA_8002: 16370 - KOG0132 RNA polymerase II C-terminal domain-binding protein RA4, contains RPR and RRM domains, at 186,103 to 188,392 16370
Group Condition mRNA_7998 mRNA_7999 mRNA_8000 mRNA_8001 mRNA_8002
16366 16367 16368 16369 16370
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.6 -0.6 +0.4 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.3 +0.1 -0.7 -0.2
growth on oleic acid YNB Glucose N.D. -1.0 +0.0 -0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.9 -0.1 -0.0 +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -1.7 +0.3 +0.1 +0.3
auxotrophy YNB Glucose N.D. -0.6 -0.1 -0.1 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. -0.4 -0.2 -0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 +0.2 -0.1 -0.2
auxotrophy Dropout Complete N.D. -0.4 -0.0 +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.4 -0.0 +0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.5 -0.3 -0.2 +0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.0 +0.2 -0.0 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 -0.0 -0.3 +0.5
growth on ricinoleic acid YNB Glucose N.D. -0.1 +0.0 -0.1 +0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 +0.0 -0.2 +0.1
growth on oleic acid YNB Oleic Acid N.D. -0.3 +0.0 -0.1 +0.6
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 -0.1 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.6 -0.0 +0.2 -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 -0.0 +0.3 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.0 -0.0 +1.0 +0.5
growth on ypd YPD N.D. +1.4 +0.1 +0.1 +0.0
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