Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16389 and 16390 are separated by 111 nucleotides16390 and 16391 are separated by 123 nucleotides16391 and 16392 are separated by 165 nucleotides16392 and 16393 are separated by 486 nucleotides mRNA_8021: 16389 - HMMPfam-NAD(P)-binding Rossmann-like domain-PF13450,SUPERFAMILY--SSF51905, at 227,452 to 229,516 16389 mRNA_8022: 16390 - BLAST Ubiquinone biosynthesis protein coq9, mitochondrial [..., at 229,628 to 230,640 16390 mRNA_8023: 16391 - HMMPfam-WD domain, G-beta repeat-PF00400,ProSitePatterns-Trp-Asp (WD) repeats signature.-PS00678,ProSiteProfiles-Trp-Asp (WD) repeats profile.-PS50082,ProSiteProfiles-Trp-Asp (WD) repeats circular profile.-PS50294,SMART-WD40 repeats-SM00320,SUPERFAMILY--SSF50978, at 230,764 to 233,185 16391 mRNA_8024: 16392 - K02988 RP-S5, MRPS5, rpsE small subunit ribosomal protein S5, at 233,351 to 234,544 16392 mRNA_8025: 16393 - KOG0118 FOG RRM domain, at 235,031 to 239,261 16393
Group Condition mRNA_8021 mRNA_8022 mRNA_8023 mRNA_8024 mRNA_8025
16389 16390 16391 16392 16393
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. -0.7 N.D. -0.5
ypd buoyancy 40 hrs Median Buoyancy -0.3 N.D. -0.3 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. -0.3 N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 N.D. -0.2 N.D. -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 N.D. -0.0 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.3 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. +0.1 N.D. -0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. -0.2 N.D. -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. +0.0 N.D. -0.3
growth on oleic acid YNB Glucose +0.0 N.D. -0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. +0.1 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. -0.1 N.D. -0.1
auxotrophy YNB Glucose +0.1 N.D. -0.2 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. -0.1 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. -0.3 N.D. -0.2
growth on ypd YPD -0.0 N.D. +0.0 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. +0.3 N.D. -0.4
auxotrophy Dropout Complete +0.2 N.D. -0.0 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. -0.2 N.D. +0.0
growth on oleic acid YNB Oleic Acid +0.0 N.D. +0.1 N.D. +0.0
growth on ricinoleic acid YNB Glucose +0.2 N.D. -0.1 N.D. +0.1
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