Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16390 and 16391 are separated by 123 nucleotides16391 and 16392 are separated by 165 nucleotides16392 and 16393 are separated by 486 nucleotides16393 and 16394 are separated by 311 nucleotides mRNA_8022: 16390 - BLAST Ubiquinone biosynthesis protein coq9, mitochondrial [..., at 229,628 to 230,640 16390 mRNA_8023: 16391 - HMMPfam-WD domain, G-beta repeat-PF00400,ProSitePatterns-Trp-Asp (WD) repeats signature.-PS00678,ProSiteProfiles-Trp-Asp (WD) repeats profile.-PS50082,ProSiteProfiles-Trp-Asp (WD) repeats circular profile.-PS50294,SMART-WD40 repeats-SM00320,SUPERFAMILY--SSF50978, at 230,764 to 233,185 16391 mRNA_8024: 16392 - K02988 RP-S5, MRPS5, rpsE small subunit ribosomal protein S5, at 233,351 to 234,544 16392 mRNA_8025: 16393 - KOG0118 FOG RRM domain, at 235,031 to 239,261 16393 mRNA_8026: 16394 - K03515 REV1 DNA repair protein REV1, at 239,573 to 243,561 16394
Group Condition mRNA_8022 mRNA_8023 mRNA_8024 mRNA_8025 mRNA_8026
16390 16391 16392 16393 16394
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.7 N.D. -0.5 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 N.D. -0.3 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.2 N.D. -0.6 +0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 N.D. -0.5 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 N.D. -0.3 +0.0
growth on oleic acid YNB Glucose N.D. -0.1 N.D. -0.3 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 N.D. -0.4 +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 N.D. -0.2 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.3 N.D. -0.1 +0.2
growth on ypd YPD N.D. +0.0 N.D. -0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 N.D. -0.2 +0.0
auxotrophy YNB Glucose N.D. -0.2 N.D. -0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.3 N.D. -0.1 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 N.D. -0.4 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 N.D. -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 N.D. +0.0 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 N.D. -0.1 +0.1
growth on oleic acid YNB Oleic Acid N.D. +0.1 N.D. +0.0 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 N.D. -0.1 +0.2
growth on ricinoleic acid YNB Glucose N.D. -0.1 N.D. +0.1 +0.2
auxotrophy Dropout Complete N.D. -0.0 N.D. -0.1 +0.4
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