Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16395 and 16396 are separated by 11 nucleotides16396 and 16397 are separated by 105 nucleotides16397 and 16398 are separated by 491 nucleotides16398 and 16399 are separated by 283 nucleotides mRNA_8027: 16395 - HMMPfam-MYND finger-PF01753,ProSiteProfiles-Zinc finger MYND-type profile.-PS50865,SUPERFAMILY--SSF144232, at 243,826 to 244,717 16395 mRNA_8028: 16396 - K06100 SYMPK symplekin, at 244,729 to 248,815 16396 mRNA_8029: 16397 - K02152 ATPeV1G, ATP6G V-type H+-transporting ATPase subunit G, at 248,921 to 249,552 16397 mRNA_8030: 16398 - KOG0116 RasGAP SH3 binding protein rasputin, contains NTF2 and RRM domains, at 250,044 to 251,923 16398 mRNA_8031: 16399 - K14325 RNPS1 RNA-binding protein with serine-rich domain 1, at 252,207 to 252,830 16399
Group Condition mRNA_8027 mRNA_8028 mRNA_8029 mRNA_8030 mRNA_8031
16395 16396 16397 16398 16399
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. N.D. -1.8 -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. N.D. -1.7 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.0 N.D. N.D. -1.1 -0.7
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 N.D. N.D. -1.0 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. N.D. -0.6 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.7 N.D. N.D. +0.5 -0.2
growth on ypd YPD -0.2 N.D. N.D. -0.5 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. N.D. -0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. N.D. -0.4 +0.5
growth on oleic acid YNB Glucose -0.2 N.D. N.D. +0.2 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. -0.4 +0.1
auxotrophy YNB Glucose -0.2 N.D. N.D. +0.3 -0.0
growth on ricinoleic acid YNB Glucose -0.2 N.D. N.D. +0.2 +0.2
auxotrophy Dropout Complete -0.0 N.D. N.D. +0.4 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. N.D. +0.3 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. N.D. -0.0 +0.5
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. N.D. +0.5 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 N.D. N.D. +0.6 +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 N.D. N.D. +0.8 +0.5
growth on oleic acid YNB Oleic Acid -0.0 N.D. N.D. +0.9 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. N.D. +0.8 +0.8
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