Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16398 and 16399 are separated by 283 nucleotides16399 and 16400 are separated by 592 nucleotides16400 and 16401 are separated by 265 nucleotides16401 and 16402 are separated by 13570 nucleotides mRNA_8030: 16398 - KOG0116 RasGAP SH3 binding protein rasputin, contains NTF2 and RRM domains, at 250,044 to 251,923 16398 mRNA_8031: 16399 - K14325 RNPS1 RNA-binding protein with serine-rich domain 1, at 252,207 to 252,830 16399 mRNA_8032: 16400 - K14007 SEC24 protein transport protein SEC24, at 253,423 to 257,120 16400 mRNA_8033: 16401 - K08488 STX7 syntaxin 7, at 257,386 to 258,889 16401 mRNA_8034: 16402 - K00898 PDK2_3_4 pyruvate dehydrogenase kinase 2/3/4, at 272,460 to 275,670 16402
Group Condition mRNA_8030 mRNA_8031 mRNA_8032 mRNA_8033 mRNA_8034
16398 16399 16400 16401 16402
facs on lipid accumulation Sorted Low BODIPY Gate -1.8 -0.4 N.D. N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate -1.1 -0.7 N.D. N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate -1.7 -0.0 N.D. N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -1.0 -0.0 N.D. N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.6 -0.1 N.D. N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.1 N.D. N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy -0.4 +0.1 N.D. N.D. +0.0
growth on ypd YPD -0.5 +0.3 N.D. N.D. +0.2
growth on oleic acid YNB Glucose +0.2 -0.2 N.D. N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.4 +0.5 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 -0.2 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 +0.5 N.D. N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine +0.3 +0.1 N.D. N.D. -0.2
auxotrophy YNB Glucose +0.3 -0.0 N.D. N.D. -0.0
auxotrophy Dropout Complete +0.4 -0.1 N.D. N.D. +0.2
growth on ricinoleic acid YNB Glucose +0.2 +0.2 N.D. N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine +0.5 +0.3 N.D. N.D. +0.0
growth on oleic acid YNB Oleic Acid +0.9 +0.3 N.D. N.D. -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.8 +0.5 N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 +0.5 N.D. N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 +0.8 N.D. N.D. -0.1
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