Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16399 and 16400 are separated by 592 nucleotides16400 and 16401 are separated by 265 nucleotides16401 and 16402 are separated by 13570 nucleotides16402 and 16403 are separated by 259 nucleotides mRNA_8031: 16399 - K14325 RNPS1 RNA-binding protein with serine-rich domain 1, at 252,207 to 252,830 16399 mRNA_8032: 16400 - K14007 SEC24 protein transport protein SEC24, at 253,423 to 257,120 16400 mRNA_8033: 16401 - K08488 STX7 syntaxin 7, at 257,386 to 258,889 16401 mRNA_8034: 16402 - K00898 PDK2_3_4 pyruvate dehydrogenase kinase 2/3/4, at 272,460 to 275,670 16402 mRNA_8035: 16403 - KOG4350 Uncharacterized conserved protein, contains BTB/POZ domain, at 275,930 to 278,853 16403
Group Condition mRNA_8031 mRNA_8032 mRNA_8033 mRNA_8034 mRNA_8035
16399 16400 16401 16402 16403
facs on lipid accumulation Sorted No BODIPY Gate -0.7 N.D. N.D. +0.1 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 N.D. N.D. +0.0 -0.6
auxotrophy YNB Glucose -0.0 N.D. N.D. -0.0 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. N.D. +0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. N.D. -0.2 -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. N.D. +0.1 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. N.D. -0.3 -0.1
growth on oleic acid YNB Oleic Acid +0.3 N.D. N.D. -0.4 -0.1
growth on oleic acid YNB Glucose -0.2 N.D. N.D. -0.1 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. N.D. -0.1 +0.2
auxotrophy Dropout Complete -0.1 N.D. N.D. +0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. N.D. +0.0 -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. N.D. -0.0 +0.3
growth on ricinoleic acid YNB Glucose +0.2 N.D. N.D. +0.0 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. N.D. -0.2 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 N.D. N.D. -0.1 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 N.D. N.D. -0.3 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. +0.0 +0.4
ypd buoyancy 40 hrs Median Buoyancy +0.5 N.D. N.D. +0.1 +0.1
growth on ypd YPD +0.3 N.D. N.D. +0.2 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 N.D. N.D. -0.0 +0.8
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