Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16400 and 16401 are separated by 265 nucleotides16401 and 16402 are separated by 13570 nucleotides16402 and 16403 are separated by 259 nucleotides16403 and 16404 are separated by 550 nucleotides mRNA_8032: 16400 - K14007 SEC24 protein transport protein SEC24, at 253,423 to 257,120 16400 mRNA_8033: 16401 - K08488 STX7 syntaxin 7, at 257,386 to 258,889 16401 mRNA_8034: 16402 - K00898 PDK2_3_4 pyruvate dehydrogenase kinase 2/3/4, at 272,460 to 275,670 16402 mRNA_8035: 16403 - KOG4350 Uncharacterized conserved protein, contains BTB/POZ domain, at 275,930 to 278,853 16403 mRNA_8036: 16404 - K01942 HLCS biotin--protein ligase, at 279,404 to 281,646 16404
Group Condition mRNA_8032 mRNA_8033 mRNA_8034 mRNA_8035 mRNA_8036
16400 16401 16402 16403 16404
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.0 -0.6 -2.3
auxotrophy YNB Glucose N.D. N.D. -0.0 -0.5 -1.2
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.0 +0.0 -1.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.2 -0.4 -0.6
growth on oleic acid YNB Glucose N.D. N.D. -0.1 +0.0 -1.0
auxotrophy Dropout Complete N.D. N.D. +0.2 -0.2 -1.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.3 +0.2 -0.7
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.3 -0.1 -0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.4 -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.0 -0.4 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.1 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.1 -0.3 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.0 +0.8 -0.9
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.1 -0.4 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.1 -0.4 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.2 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 +0.2 +0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.1 +0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.0 +0.4 +0.3
growth on ypd YPD N.D. N.D. +0.2 +0.6 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.0 +0.3 +0.7
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