Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16403 and 16404 are separated by 550 nucleotides16404 and 16405 are separated by 171 nucleotides16405 and 16406 are separated by 172 nucleotides16406 and 16407 are separated by 92 nucleotides mRNA_8035: 16403 - KOG4350 Uncharacterized conserved protein, contains BTB/POZ domain, at 275,930 to 278,853 16403 mRNA_8036: 16404 - K01942 HLCS biotin--protein ligase, at 279,404 to 281,646 16404 mRNA_8037: 16405 - K14797 ENP1, BYSL essential nuclear protein 1, at 281,818 to 283,593 16405 mRNA_8038: 16406 - K05757 ARPC1A_B actin related protein 2/3 complex, subunit 1A/1B, at 283,766 to 285,253 16406 mRNA_8039: 16407 - HMMPfam-DASH complex subunit Dad3-PF08656, at 285,346 to 285,719 16407
Group Condition mRNA_8035 mRNA_8036 mRNA_8037 mRNA_8038 mRNA_8039
16403 16404 16405 16406 16407
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 -2.3 N.D. N.D. N.D.
auxotrophy YNB Glucose -0.5 -1.2 N.D. N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.0 -1.3 N.D. N.D. N.D.
auxotrophy Dropout Complete -0.2 -1.0 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.4 -0.6 N.D. N.D. N.D.
growth on oleic acid YNB Glucose +0.0 -1.0 N.D. N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.7 N.D. N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.1 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.1 N.D. N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.2 N.D. N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.4 +0.2 N.D. N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.4 +0.3 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.8 -0.9 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.3 N.D. N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.1 +0.1 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.1 N.D. N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.2 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.4 N.D. N.D. N.D.
growth on ypd YPD +0.6 +0.1 N.D. N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.4 +0.3 N.D. N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.7 N.D. N.D. N.D.
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