Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16409 and 16410 are separated by 236 nucleotides16410 and 16411 are separated by 211 nucleotides16411 and 16412 are separated by 271 nucleotides16412 and 16413 are separated by 334 nucleotides mRNA_8041: 16409 - K13350 PXMP4, PMP24 peroxisomal membrane protein 4, at 287,723 to 288,731 16409 mRNA_8042: 16410 - K17408 DAP3, MRPS29 small subunit ribosomal protein S29, at 288,968 to 290,792 16410 mRNA_8043: 16411 - K15283 SLC35E1 solute carrier family 35, member E1, at 291,004 to 293,289 16411 mRNA_8044: 16412 - KOG4520 Predicted coiled-coil protein, at 293,561 to 294,571 16412 mRNA_8045: 16413 - Hypothetical Protein, at 294,906 to 295,366 16413
Group Condition mRNA_8041 mRNA_8042 mRNA_8043 mRNA_8044 mRNA_8045
16409 16410 16411 16412 16413
facs on lipid accumulation Sorted High BODIPY Gate -1.1 N.D. +0.4 N.D. -0.3
facs on lipid accumulation Sorted No BODIPY Gate -1.3 N.D. +0.6 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -1.2 N.D. +0.5 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 N.D. -0.8 N.D. -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 N.D. +0.2 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine -0.6 N.D. +0.2 N.D. +0.1
growth on ypd YPD -0.1 N.D. -0.1 N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -1.6 N.D. +1.4 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. -0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. +0.0 N.D. -0.1
growth on ricinoleic acid YNB Glucose -0.4 N.D. +0.3 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. +0.1 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.4 N.D. -0.3 N.D. -0.0
auxotrophy YNB Glucose -0.4 N.D. +0.3 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 N.D. -0.2 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -1.3 N.D. +1.4 N.D. +0.3
growth on oleic acid YNB Glucose -0.1 N.D. +0.4 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.6 N.D. +0.2 N.D. +0.1
growth on oleic acid YNB Oleic Acid +0.1 N.D. +0.8 N.D. +0.2
auxotrophy Dropout Complete +0.5 N.D. +0.5 N.D. +0.3
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