Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16427 and 16428 are separated by 715 nucleotides16428 and 16429 are separated by 868 nucleotides16429 and 16430 are separated by 152 nucleotides16430 and 16431 are separated by 507 nucleotides mRNA_8059: 16427 - HMMPfam-Translation initiation factor IF-3, C-terminal domain-PF00707,HMMPfam-Translation initiation factor IF-3, N-terminal domain-PF05198,SUPERFAMILY--SSF54364,SUPERFAMILY--SSF55200, at 324,105 to 324,902 16427 mRNA_8060: 16428 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 325,618 to 328,246 16428 mRNA_8061: 16429 - BLAST protein of unknown function [Taphrina deformans PYCC ..., at 329,115 to 329,494 16429 mRNA_8062: 16430 - KOG1441 Glucose-6-phosphate/phosphate and phosphoenolpyruvate/phosphate antiporter, at 329,647 to 331,079 16430 mRNA_8063: 16431 - HMMPfam-Protein of unknown function (DUF3500)-PF12006, at 331,587 to 333,110 16431
Group Condition mRNA_8059 mRNA_8060 mRNA_8061 mRNA_8062 mRNA_8063
16427 16428 16429 16430 16431
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -1.2 N.D. -0.2 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.5 N.D. +0.4 -0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.5 N.D. +0.1 -0.3
growth on ypd YPD N.D. -0.2 N.D. -0.5 +0.2
growth on oleic acid YNB Oleic Acid N.D. -0.1 N.D. -0.6 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 N.D. -0.0 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.0 N.D. -0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 N.D. -0.1 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 N.D. -0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 N.D. -0.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 N.D. -0.4 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 N.D. -0.1 +0.0
auxotrophy Dropout Complete N.D. -0.1 N.D. +0.0 +0.1
growth on oleic acid YNB Glucose N.D. -0.0 N.D. -0.1 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 N.D. -0.0 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 N.D. +0.0 +0.2
auxotrophy YNB Glucose N.D. -0.1 N.D. -0.0 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 N.D. +0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 N.D. +0.0 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.5 N.D. -0.5 +0.2
growth on ricinoleic acid YNB Glucose N.D. +0.2 N.D. -0.1 +0.3
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