Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16431 and 16432 are separated by 296 nucleotides16432 and 16433 are separated by 94 nucleotides16433 and 16434 are separated by 685 nucleotides16434 and 16435 are separated by 711 nucleotides mRNA_8063: 16431 - HMMPfam-Protein of unknown function (DUF3500)-PF12006, at 331,587 to 333,110 16431 mRNA_8064: 16432 - HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 333,407 to 335,918 16432 mRNA_8065: 16433 - KOG0732 AAA+-type ATPase containing the bromodomain, at 336,013 to 341,106 16433 mRNA_8066: 16434 - K20181 VPS18, PEP3 vacuolar protein sorting-associated protein 18, at 341,792 to 345,856 16434 mRNA_8067: 16435 - K12399 AP3S AP-3 complex subunit sigma, at 346,568 to 347,476 16435
Group Condition mRNA_8063 mRNA_8064 mRNA_8065 mRNA_8066 mRNA_8067
16431 16432 16433 16434 16435
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 -0.6 N.D. N.D. -2.7
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.1 N.D. N.D. -1.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.3 N.D. N.D. -0.9
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.0 N.D. N.D. +0.1
growth on oleic acid YNB Glucose +0.2 -0.1 N.D. N.D. -0.3
growth on ypd YPD +0.2 +0.0 N.D. N.D. -0.4
growth on oleic acid YNB Oleic Acid +0.2 +0.1 N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.1 N.D. N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.0 N.D. N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.1 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.2 N.D. N.D. +0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 +0.1 N.D. N.D. +0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.1 N.D. N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.1 N.D. N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.0 N.D. N.D. +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.2 N.D. N.D. +0.6
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.2 N.D. N.D. +0.2
auxotrophy YNB Glucose +0.3 -0.1 N.D. N.D. +0.5
growth on ricinoleic acid YNB Glucose +0.3 +0.2 N.D. N.D. +0.2
auxotrophy Dropout Complete +0.1 +0.1 N.D. N.D. +1.0
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.0 N.D. N.D. +1.0
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