Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16432 and 16433 are separated by 94 nucleotides16433 and 16434 are separated by 685 nucleotides16434 and 16435 are separated by 711 nucleotides16435 and 16436 are separated by 257 nucleotides mRNA_8064: 16432 - HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 333,407 to 335,918 16432 mRNA_8065: 16433 - KOG0732 AAA+-type ATPase containing the bromodomain, at 336,013 to 341,106 16433 mRNA_8066: 16434 - K20181 VPS18, PEP3 vacuolar protein sorting-associated protein 18, at 341,792 to 345,856 16434 mRNA_8067: 16435 - K12399 AP3S AP-3 complex subunit sigma, at 346,568 to 347,476 16435 mRNA_8068: 16436 - KOG1198 Zinc-binding oxidoreductase, at 347,734 to 349,027 16436
Group Condition mRNA_8064 mRNA_8065 mRNA_8066 mRNA_8067 mRNA_8068
16432 16433 16434 16435 16436
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 N.D. N.D. -2.7 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. N.D. -1.2 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. N.D. -0.9 -0.3
growth on oleic acid YNB Glucose -0.1 N.D. N.D. -0.3 -0.3
growth on oleic acid YNB Oleic Acid +0.1 N.D. N.D. -0.3 -0.4
growth on ypd YPD +0.0 N.D. N.D. -0.4 -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. N.D. +0.0 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. N.D. +0.6 -0.5
auxotrophy YNB Glucose -0.1 N.D. N.D. +0.5 -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. N.D. +0.2 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. N.D. +0.2 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. N.D. +0.1 +0.1
growth on ricinoleic acid YNB Glucose +0.2 N.D. N.D. +0.2 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. N.D. +0.3 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. +0.2 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. N.D. +0.6 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. N.D. +0.7 +0.1
auxotrophy Dropout Complete +0.1 N.D. N.D. +1.0 -0.3
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. N.D. +1.0 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.5 +0.3
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