Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16434 and 16435 are separated by 711 nucleotides16435 and 16436 are separated by 257 nucleotides16436 and 16437 are separated by 238 nucleotides16437 and 16438 are separated by 733 nucleotides mRNA_8066: 16434 - K20181 VPS18, PEP3 vacuolar protein sorting-associated protein 18, at 341,792 to 345,856 16434 mRNA_8067: 16435 - K12399 AP3S AP-3 complex subunit sigma, at 346,568 to 347,476 16435 mRNA_8068: 16436 - KOG1198 Zinc-binding oxidoreductase, at 347,734 to 349,027 16436 mRNA_8069: 16437 - K11240 MSG5 tyrosine-protein phosphatase MSG5, at 349,266 to 352,237 16437 mRNA_8070: 16438 - KOG2744 DNA-binding proteins Bright/BRCAA1/RBP1 and related proteins containing BRIGHT domain, at 352,971 to 356,378 16438
Group Condition mRNA_8066 mRNA_8067 mRNA_8068 mRNA_8069 mRNA_8070
16434 16435 16436 16437 16438
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -2.7 -0.2 +0.1 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -1.2 -0.5 +1.0 -0.4
growth on oleic acid YNB Oleic Acid N.D. -0.3 -0.4 +0.3 -0.1
growth on oleic acid YNB Glucose N.D. -0.3 -0.3 +0.4 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 -0.2 +0.0 -0.3
growth on ypd YPD N.D. -0.4 -0.0 +0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 -0.2 -0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.9 -0.3 +1.0 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 -0.1 -0.1 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 -0.2 -0.1 +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.6 -0.5 +0.3 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 +0.1 +0.5 -0.5
auxotrophy YNB Glucose N.D. +0.5 -0.3 +0.6 -0.5
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 +0.0 +0.4 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 +0.1 +0.3 -0.0
growth on ricinoleic acid YNB Glucose N.D. +0.2 -0.3 +0.7 -0.1
auxotrophy Dropout Complete N.D. +1.0 -0.3 +0.2 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +1.0 -0.3 +0.4 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.5 +0.3 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.7 +0.1 +0.3 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.6 +0.0 +0.2 +0.7
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