Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16438 and 16439 are separated by 782 nucleotides16439 and 16440 are separated by 365 nucleotides16440 and 16441 are separated by 405 nucleotides16441 and 16442 are separated by 409 nucleotides mRNA_8070: 16438 - KOG2744 DNA-binding proteins Bright/BRCAA1/RBP1 and related proteins containing BRIGHT domain, at 352,971 to 356,378 16438 mRNA_8071: 16439 - Hypothetical Protein, at 357,161 to 359,241 16439 mRNA_8072: 16440 - K14684 SLC25A23S solute carrier family 25 (mitochondrial phosphate transporter), member 23/24/25/41, at 359,607 to 361,273 16440 mRNA_8073: 16441 - Hypothetical Protein, at 361,679 to 362,906 16441 mRNA_8074: 16442 - Hypothetical Protein, at 363,316 to 363,918 16442
Group Condition mRNA_8070 mRNA_8071 mRNA_8072 mRNA_8073 mRNA_8074
16438 16439 16440 16441 16442
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -0.8 +0.1 +0.1 -0.8
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 -0.9 +0.4 +0.1 -1.3
growth on oleic acid YNB Oleic Acid -0.1 -0.1 -0.6 -0.1 -0.3
growth on oleic acid YNB Glucose +0.0 -0.2 -0.6 -0.3 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 +0.4 +0.0 -0.2 -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.0 -0.6 +0.5 +0.2 -0.5
auxotrophy YNB Glucose -0.5 +0.0 +0.6 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.5 +0.9 -0.2 -0.6
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.1 -0.1 +0.0 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 -0.1 +0.7 -0.3 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.1 +0.3 -0.3 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.3 +0.1 -0.3 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.0 -0.0 -0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.2 -0.0 -0.1 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.2 +0.3 -0.4 +0.5
growth on ypd YPD +0.2 -0.0 +0.6 -0.0 -0.2
growth on ricinoleic acid YNB Glucose -0.1 +0.3 +0.2 -0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.4 +0.6 -0.3 -0.5
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.4 -0.1 -0.0 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.7 -0.5 +0.6 +0.2 -0.2
auxotrophy Dropout Complete +0.0 +0.1 +0.8 -0.0 +0.0
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