Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16440 and 16441 are separated by 405 nucleotides16441 and 16442 are separated by 409 nucleotides16442 and 16443 are separated by 409 nucleotides16443 and 16444 are separated by 1203 nucleotides mRNA_8072: 16440 - K14684 SLC25A23S solute carrier family 25 (mitochondrial phosphate transporter), member 23/24/25/41, at 359,607 to 361,273 16440 mRNA_8073: 16441 - Hypothetical Protein, at 361,679 to 362,906 16441 mRNA_8074: 16442 - Hypothetical Protein, at 363,316 to 363,918 16442 mRNA_8075: 16443 - K13525 VCP, CDC48 transitional endoplasmic reticulum ATPase, at 364,328 to 367,178 16443 mRNA_8076: 16444 - Hypothetical Protein, at 368,382 to 369,179 16444
Group Condition mRNA_8072 mRNA_8073 mRNA_8074 mRNA_8075 mRNA_8076
16440 16441 16442 16443 16444
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 +0.1 -1.3 N.D. -0.6
growth on oleic acid YNB Oleic Acid -0.6 -0.1 -0.3 N.D. -0.1
growth on oleic acid YNB Glucose -0.6 -0.3 +0.0 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 -0.2 -0.5 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.1 -0.8 N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 -0.3 -0.2 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.3 +0.1 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 -0.3 -0.5 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.9 -0.2 -0.6 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 -0.1 +0.2 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.5 +0.2 -0.5 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.1 +0.3 N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.0 +0.3 N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.0 +0.4 N.D. -0.0
auxotrophy YNB Glucose +0.6 -0.2 -0.1 N.D. -0.1
growth on ypd YPD +0.6 -0.0 -0.2 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine +0.3 -0.4 +0.5 N.D. -0.1
growth on ricinoleic acid YNB Glucose +0.2 -0.1 +0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.7 -0.3 -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 +0.2 -0.2 N.D. -0.2
auxotrophy Dropout Complete +0.8 -0.0 +0.0 N.D. -0.1
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