Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16441 and 16442 are separated by 409 nucleotides16442 and 16443 are separated by 409 nucleotides16443 and 16444 are separated by 1203 nucleotides16444 and 16445 are separated by 359 nucleotides mRNA_8073: 16441 - Hypothetical Protein, at 361,679 to 362,906 16441 mRNA_8074: 16442 - Hypothetical Protein, at 363,316 to 363,918 16442 mRNA_8075: 16443 - K13525 VCP, CDC48 transitional endoplasmic reticulum ATPase, at 364,328 to 367,178 16443 mRNA_8076: 16444 - Hypothetical Protein, at 368,382 to 369,179 16444 mRNA_8077: 16445 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 369,539 to 370,935 16445
Group Condition mRNA_8073 mRNA_8074 mRNA_8075 mRNA_8076 mRNA_8077
16441 16442 16443 16444 16445
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -1.3 N.D. -0.6 -2.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.8 N.D. +0.0 -0.7
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.5 N.D. -0.1 -0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.6 N.D. -0.1 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.5 N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.2 N.D. -0.2 +0.1
growth on oleic acid YNB Oleic Acid -0.1 -0.3 N.D. -0.1 -0.1
auxotrophy YNB Glucose -0.2 -0.1 N.D. -0.1 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -0.5 N.D. +0.1 +0.1
growth on oleic acid YNB Glucose -0.3 +0.0 N.D. -0.1 -0.1
growth on ypd YPD -0.0 -0.2 N.D. -0.1 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.2 N.D. -0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.1 N.D. -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.4 +0.5 N.D. -0.1 -0.2
auxotrophy Dropout Complete -0.0 +0.0 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 -0.1 N.D. -0.1 +0.2
growth on ricinoleic acid YNB Glucose -0.1 +0.1 N.D. +0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.2 N.D. +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.3 N.D. +0.0 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.3 N.D. +0.0 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.4 N.D. -0.0 +0.1
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