Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16442 and 16443 are separated by 409 nucleotides16443 and 16444 are separated by 1203 nucleotides16444 and 16445 are separated by 359 nucleotides16445 and 16446 are separated by 194 nucleotides mRNA_8074: 16442 - Hypothetical Protein, at 363,316 to 363,918 16442 mRNA_8075: 16443 - K13525 VCP, CDC48 transitional endoplasmic reticulum ATPase, at 364,328 to 367,178 16443 mRNA_8076: 16444 - Hypothetical Protein, at 368,382 to 369,179 16444 mRNA_8077: 16445 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 369,539 to 370,935 16445 mRNA_8078: 16446 - HMMPfam-ER membrane protein SH3-PF08229,SMART-ER membrane protein SH3-SM00786, at 371,130 to 372,195 16446
Group Condition mRNA_8074 mRNA_8075 mRNA_8076 mRNA_8077 mRNA_8078
16442 16443 16444 16445 16446
facs on lipid accumulation Sorted Low BODIPY Gate -1.3 N.D. -0.6 -2.2 -1.9
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 N.D. +0.1 +0.1 -1.2
facs on lipid accumulation Sorted No BODIPY Gate -0.5 N.D. -0.1 -0.6 +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.8 N.D. +0.0 -0.7 +0.3
growth on ypd YPD -0.2 N.D. -0.1 -0.0 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 N.D. +0.1 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.6 N.D. -0.1 -0.0 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. -0.2 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. -0.1 +0.2 -0.8
growth on oleic acid YNB Oleic Acid -0.3 N.D. -0.1 -0.1 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. -0.2 -0.1 +0.3
growth on oleic acid YNB Glucose +0.0 N.D. -0.1 -0.1 -0.1
auxotrophy YNB Glucose -0.1 N.D. -0.1 -0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. +0.1 -0.2 -0.3
growth on ricinoleic acid YNB Glucose +0.1 N.D. +0.1 -0.2 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.5 N.D. -0.1 -0.2 -0.1
auxotrophy Dropout Complete +0.0 N.D. -0.1 -0.1 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. -0.1 -0.1 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.3 N.D. +0.0 +0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. +0.0 +0.1 +0.5
ypd buoyancy 40 hrs Low Buoyancy +0.4 N.D. -0.0 +0.1 +0.5
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