Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16443 and 16444 are separated by 1203 nucleotides16444 and 16445 are separated by 359 nucleotides16445 and 16446 are separated by 194 nucleotides16446 and 16447 are separated by 260 nucleotides mRNA_8075: 16443 - K13525 VCP, CDC48 transitional endoplasmic reticulum ATPase, at 364,328 to 367,178 16443 mRNA_8076: 16444 - Hypothetical Protein, at 368,382 to 369,179 16444 mRNA_8077: 16445 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 369,539 to 370,935 16445 mRNA_8078: 16446 - HMMPfam-ER membrane protein SH3-PF08229,SMART-ER membrane protein SH3-SM00786, at 371,130 to 372,195 16446 mRNA_8079: 16447 - BLAST PRMT5-domain-containing protein [Sanghuangporus baumii], at 372,456 to 373,383 16447
Group Condition mRNA_8075 mRNA_8076 mRNA_8077 mRNA_8078 mRNA_8079
16443 16444 16445 16446 16447
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.6 -2.2 -1.9 -0.5
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 +0.1 -1.2 -0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 +0.2 -0.8 -0.5
growth on ypd YPD N.D. -0.1 -0.0 -0.4 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 -0.6 +0.1 +0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.0 -0.7 +0.3 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 +0.1 -0.3 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 -0.2 -0.3 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 -0.2 -0.1 +0.1
growth on ricinoleic acid YNB Glucose N.D. +0.1 -0.2 -0.0 -0.2
auxotrophy YNB Glucose N.D. -0.1 -0.2 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 -0.0 +0.1 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 -0.1 +0.3 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 -0.1 -0.2 +0.2
growth on oleic acid YNB Oleic Acid N.D. -0.1 -0.1 +0.0 +0.1
growth on oleic acid YNB Glucose N.D. -0.1 -0.1 -0.1 +0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 +0.0 +0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 -0.1 +0.3 +0.2
auxotrophy Dropout Complete N.D. -0.1 -0.1 +0.4 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 +0.1 +0.5 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 +0.1 +0.5 +0.3
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