Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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Top 21 experiments (either direction), sorted by average fitness

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500 nt 16445 and 16446 are separated by 194 nucleotides16446 and 16447 are separated by 260 nucleotides16447 and 16448 are separated by 168 nucleotides16448 and 16449 are separated by 175 nucleotides mRNA_8077: 16445 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 369,539 to 370,935 16445 mRNA_8078: 16446 - HMMPfam-ER membrane protein SH3-PF08229,SMART-ER membrane protein SH3-SM00786, at 371,130 to 372,195 16446 mRNA_8079: 16447 - BLAST PRMT5-domain-containing protein [Sanghuangporus baumii], at 372,456 to 373,383 16447 mRNA_8080: 16448 - HMMPfam-Alpha/beta hydrolase family-PF12697,SUPERFAMILY--SSF53474, at 373,552 to 376,166 16448 mRNA_8081: 16449 - K00905 BCKDK [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase, at 376,342 to 377,975 16449
Group Condition mRNA_8077 mRNA_8078 mRNA_8079 mRNA_8080 mRNA_8081
16445 16446 16447 16448 16449
facs on lipid accumulation Sorted Low BODIPY Gate -2.2 -1.9 -0.5 -0.1 +0.5
growth on oleic acid YNB Oleic Acid -0.1 +0.0 +0.1 -0.3 -1.9
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.2 +0.2 -0.1 -1.8
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.3 +0.1 +0.2 -1.6
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -1.2 -0.7 +0.4 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.7 +0.3 -0.1 -0.0 -0.7
growth on oleic acid YNB Glucose -0.1 -0.1 +0.5 -0.2 -1.2
auxotrophy YNB Glucose -0.2 +0.1 +0.1 -0.4 -0.4
growth on ricinoleic acid YNB Glucose -0.2 -0.0 -0.2 +0.2 -0.6
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.5 -0.2 +0.0 -1.0
growth on ypd YPD -0.0 -0.4 -0.1 +0.5 -0.5
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.1 +0.1 -0.3 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.8 -0.5 -0.1 +0.9
auxotrophy Dropout Complete -0.1 +0.4 +0.1 +0.1 -0.6
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.1 +0.1 +0.1 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.3 +0.1 -0.1 +0.7
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.3 +0.2 -0.3 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.3 +0.1 -0.2 +0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 +0.1 -0.0 -0.3 +1.0
facs on lipid accumulation Sorted No BODIPY Gate -0.6 +0.1 +0.0 +0.1 +1.2
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.5 +0.3 +0.1 +0.1
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