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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
16445 and 16446 are separated by 194 nucleotides
16446 and 16447 are separated by 260 nucleotides
16447 and 16448 are separated by 168 nucleotides
16448 and 16449 are separated by 175 nucleotides
mRNA_8077: 16445 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 369,539 to 370,935
16445
mRNA_8078: 16446 - HMMPfam-ER membrane protein SH3-PF08229,SMART-ER membrane protein SH3-SM00786, at 371,130 to 372,195
16446
mRNA_8079: 16447 - BLAST PRMT5-domain-containing protein [Sanghuangporus baumii], at 372,456 to 373,383
16447
mRNA_8080: 16448 - HMMPfam-Alpha/beta hydrolase family-PF12697,SUPERFAMILY--SSF53474, at 373,552 to 376,166
16448
mRNA_8081: 16449 - K00905 BCKDK [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase, at 376,342 to 377,975
16449
Group
Condition
mRNA
_8077
mRNA
_8078
mRNA
_8079
mRNA
_8080
mRNA
_8081
16445
16446
16447
16448
16449
facs on lipid accumulation
Sorted Low BODIPY Gate
-2.2
-1.9
-0.5
-0.1
+0.5
growth on oleic acid
YNB Oleic Acid
-0.1
+0.0
+0.1
-0.3
-1.9
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.1
-0.2
+0.2
-0.1
-1.8
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.2
-0.3
+0.1
+0.2
-1.6
ypd buoyancy
40 hrs No Buoyancy Separation
+0.1
-1.2
-0.7
+0.4
-0.3
facs on lipid accumulation
Sorted High BODIPY Gate
-0.7
+0.3
-0.1
-0.0
-0.7
growth on oleic acid
YNB Glucose
-0.1
-0.1
+0.5
-0.2
-1.2
auxotrophy
YNB Glucose
-0.2
+0.1
+0.1
-0.4
-0.4
growth on ricinoleic acid
YNB Glucose
-0.2
-0.0
-0.2
+0.2
-0.6
ypd buoyancy
40 hrs Low Buoyancy
+0.1
+0.5
-0.2
+0.0
-1.0
growth on ypd
YPD
-0.0
-0.4
-0.1
+0.5
-0.5
auxotrophy
YNB Glucose 75mM Arginine
-0.2
-0.1
+0.1
-0.3
-0.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.2
-0.8
-0.5
-0.1
+0.9
auxotrophy
Dropout Complete
-0.1
+0.4
+0.1
+0.1
-0.6
ypd buoyancy
40 hrs Median Buoyancy
+0.0
+0.1
+0.1
+0.1
-0.0
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.1
-0.3
+0.1
-0.1
+0.7
auxotrophy
YNB Glucose 75mM Methionine
-0.1
+0.3
+0.2
-0.3
+0.5
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.1
+0.3
+0.1
-0.2
+0.6
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.0
+0.1
-0.0
-0.3
+1.0
facs on lipid accumulation
Sorted No BODIPY Gate
-0.6
+0.1
+0.0
+0.1
+1.2
ypd buoyancy
40 hrs High Buoyancy
+0.1
+0.5
+0.3
+0.1
+0.1
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