Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16446 and 16447 are separated by 260 nucleotides16447 and 16448 are separated by 168 nucleotides16448 and 16449 are separated by 175 nucleotides16449 and 16450 are separated by 477 nucleotides mRNA_8078: 16446 - HMMPfam-ER membrane protein SH3-PF08229,SMART-ER membrane protein SH3-SM00786, at 371,130 to 372,195 16446 mRNA_8079: 16447 - BLAST PRMT5-domain-containing protein [Sanghuangporus baumii], at 372,456 to 373,383 16447 mRNA_8080: 16448 - HMMPfam-Alpha/beta hydrolase family-PF12697,SUPERFAMILY--SSF53474, at 373,552 to 376,166 16448 mRNA_8081: 16449 - K00905 BCKDK [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase, at 376,342 to 377,975 16449 mRNA_8082: 16450 - BLAST zinc-binding oxidoreductase [Moesziomyces antarcticus..., at 378,453 to 379,519 16450
Group Condition mRNA_8078 mRNA_8079 mRNA_8080 mRNA_8081 mRNA_8082
16446 16447 16448 16449 16450
facs on lipid accumulation Sorted Low BODIPY Gate -1.9 -0.5 -0.1 +0.5 N.D.
growth on oleic acid YNB Oleic Acid +0.0 +0.1 -0.3 -1.9 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.2 -0.1 -1.8 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -1.2 -0.7 +0.4 -0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 +0.1 +0.2 -1.6 N.D.
growth on oleic acid YNB Glucose -0.1 +0.5 -0.2 -1.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.5 -0.2 +0.0 -1.0 N.D.
auxotrophy YNB Glucose +0.1 +0.1 -0.4 -0.4 N.D.
growth on ypd YPD -0.4 -0.1 +0.5 -0.5 N.D.
growth on ricinoleic acid YNB Glucose -0.0 -0.2 +0.2 -0.6 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -0.1 -0.0 -0.7 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.8 -0.5 -0.1 +0.9 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.1 -0.3 -0.0 N.D.
auxotrophy Dropout Complete +0.4 +0.1 +0.1 -0.6 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.1 +0.1 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.1 -0.1 +0.7 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.2 -0.3 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.1 -0.2 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.0 -0.3 +1.0 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.5 +0.3 +0.1 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.0 +0.1 +1.2 N.D.
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