Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16448 and 16449 are separated by 175 nucleotides16449 and 16450 are separated by 477 nucleotides16450 and 16451 are separated by 197 nucleotides16451 and 16452 are separated by 111 nucleotides mRNA_8080: 16448 - HMMPfam-Alpha/beta hydrolase family-PF12697,SUPERFAMILY--SSF53474, at 373,552 to 376,166 16448 mRNA_8081: 16449 - K00905 BCKDK [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase, at 376,342 to 377,975 16449 mRNA_8082: 16450 - BLAST zinc-binding oxidoreductase [Moesziomyces antarcticus..., at 378,453 to 379,519 16450 mRNA_8083: 16451 - K17774 MDM10 mitochondrial distribution and morphology protein 10, at 379,717 to 381,351 16451 mRNA_8084: 16452 - K05351 E1.1.1.9 D-xylulose reductase, at 381,463 to 383,498 16452
Group Condition mRNA_8080 mRNA_8081 mRNA_8082 mRNA_8083 mRNA_8084
16448 16449 16450 16451 16452
growth on oleic acid YNB Oleic Acid -0.3 -1.9 N.D. N.D. -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -1.8 N.D. N.D. -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -1.6 N.D. N.D. -0.4
growth on oleic acid YNB Glucose -0.2 -1.2 N.D. N.D. -0.4
auxotrophy YNB Glucose -0.4 -0.4 N.D. N.D. -0.4
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.0 N.D. N.D. -0.9
ypd buoyancy 40 hrs Low Buoyancy +0.0 -1.0 N.D. N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.5 N.D. N.D. -1.0
growth on ricinoleic acid YNB Glucose +0.2 -0.6 N.D. N.D. -0.4
auxotrophy Dropout Complete +0.1 -0.6 N.D. N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.7 N.D. N.D. +0.1
growth on ypd YPD +0.5 -0.5 N.D. N.D. -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.0 N.D. N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.1 N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 +0.6 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.7 N.D. N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 -0.3 N.D. N.D. +0.7
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.5 N.D. N.D. +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 +1.0 N.D. N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +1.2 N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.9 N.D. N.D. +0.2
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