Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16452 and 16453 are separated by 675 nucleotides16453 and 16454 are separated by 840 nucleotides16454 and 16455 are separated by 1085 nucleotides16455 and 16456 are separated by 296 nucleotides mRNA_8084: 16452 - K05351 E1.1.1.9 D-xylulose reductase, at 381,463 to 383,498 16452 mRNA_8085: 16453 - HMMPfam-Glycosyltransferase sugar-binding region containing DXD motif-PF04488,SUPERFAMILY--SSF53448, at 384,174 to 386,638 16453 mRNA_8086: 16454 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 387,479 to 389,690 16454 mRNA_8087: 16455 - ProSiteProfiles-BRCT domain profile.-PS50172,SUPERFAMILY--SSF52113, at 390,776 to 392,470 16455 mRNA_8088: 16456 - Hypothetical Protein, at 392,767 to 393,298 16456
Group Condition mRNA_8084 mRNA_8085 mRNA_8086 mRNA_8087 mRNA_8088
16452 16453 16454 16455 16456
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. -0.2 -0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.9 N.D. +0.1 -0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine -1.0 N.D. -0.0 +0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. -0.3 -0.7 N.D.
growth on ypd YPD -0.3 N.D. +0.0 -0.5 N.D.
growth on oleic acid YNB Oleic Acid -0.3 N.D. -0.0 -0.3 N.D.
growth on oleic acid YNB Glucose -0.4 N.D. -0.0 -0.2 N.D.
auxotrophy YNB Glucose -0.4 N.D. -0.3 +0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 N.D. +0.2 +0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 N.D. -0.3 -0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. -0.0 -0.1 N.D.
auxotrophy Dropout Complete -0.1 N.D. -0.1 +0.2 N.D.
growth on ricinoleic acid YNB Glucose -0.4 N.D. +0.1 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. -0.1 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. -0.4 +0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. +0.2 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. -0.3 +0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. +0.1 +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 N.D. +0.3 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. -0.3 +0.6 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.7 N.D. -0.5 +0.2 N.D.
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