Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16455 and 16456 are separated by 296 nucleotides16456 and 16457 are separated by 317 nucleotides16457 and 16458 are separated by 167 nucleotides16458 and 16459 are separated by 129 nucleotides mRNA_8087: 16455 - ProSiteProfiles-BRCT domain profile.-PS50172,SUPERFAMILY--SSF52113, at 390,776 to 392,470 16455 mRNA_8088: 16456 - Hypothetical Protein, at 392,767 to 393,298 16456 mRNA_8089: 16457 - Hypothetical Protein, at 393,616 to 394,916 16457 mRNA_8090: 16458 - K12737 SDCCAG10 peptidyl-prolyl cis-trans isomerase SDCCAG10, at 395,084 to 397,372 16458 mRNA_8091: 16459 - K15463 RIT1 tRNA A64-2'-O-ribosylphosphate transferase, at 397,502 to 399,447 16459
Group Condition mRNA_8087 mRNA_8088 mRNA_8089 mRNA_8090 mRNA_8091
16455 16456 16457 16458 16459
facs on lipid accumulation Sorted High BODIPY Gate -0.7 N.D. +0.2 -0.5 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. -0.2 -0.2 -0.3
auxotrophy YNB Glucose +0.2 N.D. -0.2 +0.1 -0.7
growth on ypd YPD -0.5 N.D. -0.4 -0.1 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. -0.2 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. -0.7 -0.4 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. +0.1 +0.2 -0.6
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. +0.1 +0.4 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. +0.3 -0.1 +0.1
growth on ricinoleic acid YNB Glucose +0.4 N.D. +0.0 +0.1 -0.5
growth on oleic acid YNB Oleic Acid -0.3 N.D. +0.3 +0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. +0.2 -0.1 -0.3
growth on oleic acid YNB Glucose -0.2 N.D. +0.2 +0.3 -0.2
auxotrophy Dropout Complete +0.2 N.D. +0.2 +0.2 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 N.D. -0.2 -0.5 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. +0.7 -0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.0 +0.4 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. -0.0 +0.3 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. +0.5 +0.2 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. -0.0 -0.1 +0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. +0.2 -0.0 +0.9
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