Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16459 and 16460 are separated by 435 nucleotides16460 and 16461 are separated by 381 nucleotides16461 and 16462 are separated by 190 nucleotides16462 and 16463 are separated by 507 nucleotides mRNA_8091: 16459 - K15463 RIT1 tRNA A64-2'-O-ribosylphosphate transferase, at 397,502 to 399,447 16459 mRNA_8092: 16460 - K14457 MOGAT2, MGAT2 2-acylglycerol O-acyltransferase 2, at 399,883 to 401,738 16460 mRNA_8093: 16461 - HMMPfam-Acetyltransferase (GNAT) family-PF00583,ProSiteProfiles-Gcn5-related N-acetyltransferase (GNAT) domain profile.-PS51186,SUPERFAMILY--SSF55729, at 402,120 to 403,069 16461 mRNA_8094: 16462 - K10775 PAL phenylalanine ammonia-lyase, at 403,260 to 405,803 16462 mRNA_8095: 16463 - K17508 PTC7, PPTC7 protein phosphatase PTC7, at 406,311 to 408,343 16463
Group Condition mRNA_8091 mRNA_8092 mRNA_8093 mRNA_8094 mRNA_8095
16459 16460 16461 16462 16463
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -1.6 -0.2 +0.1 -0.8
auxotrophy YNB Glucose -0.7 -0.0 -0.1 -0.1 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.8 +0.1 -0.3 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.1 -1.0 +0.5 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 -1.3 +0.1 +0.2 +0.1
growth on oleic acid YNB Glucose -0.2 -0.3 -0.2 -0.3 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.0 +0.1 +0.1 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.6 +0.3 +0.1 -0.0 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.2 +0.1 -0.1 +0.2
growth on ricinoleic acid YNB Glucose -0.5 +0.1 +0.2 -0.1 +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.6 +0.2 +0.1 +0.3
growth on oleic acid YNB Oleic Acid -0.1 -0.1 +0.1 -0.2 +0.2
auxotrophy Dropout Complete -0.3 +0.1 +0.5 -0.0 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 +0.1 +0.1 -0.2 +0.1
growth on ypd YPD +0.3 -0.2 +0.4 -0.4 +0.4
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.0 +0.1 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 -0.2 +0.5 -0.4 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.4 +0.1 +0.3 +0.3 +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.1 +0.5 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.9 +0.8 +0.2 -0.1 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +2.3 +0.2 -0.4 +1.2
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