Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16474 and 16475 are separated by 358 nucleotides16475 and 16476 are separated by 337 nucleotides16476 and 16477 are separated by 591 nucleotides16477 and 16478 are separated by 547 nucleotides mRNA_8106: 16474 - K13511 TAZ monolysocardiolipin acyltransferase, at 425,625 to 426,745 16474 mRNA_8107: 16475 - K01937 pyrG, CTPS CTP synthase, at 427,104 to 429,524 16475 mRNA_8108: 16476 - K17637 EXOC2, SEC5 exocyst complex component 2, at 429,862 to 433,885 16476 mRNA_8109: 16477 - K00679 E2.3.1.158 phospholipid-diacylglycerol acyltransferase, at 434,477 to 437,050 16477 mRNA_8110: 16478 - HMMPfam-G protein-coupled glucose receptor regulating Gpa2-PF11710,SUPERFAMILY--SSF81321, at 437,598 to 439,156 16478
Group Condition mRNA_8106 mRNA_8107 mRNA_8108 mRNA_8109 mRNA_8110
16474 16475 16476 16477 16478
growth on ricinoleic acid YNB Ricinoleic Acid -2.1 N.D. N.D. -0.3 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -1.5 N.D. N.D. -0.8 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 N.D. N.D. -0.6 -0.3
auxotrophy YNB Glucose 75mM Arginine -0.8 N.D. N.D. -0.7 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. N.D. -1.0 -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.5 N.D. N.D. -0.5 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. N.D. -0.8 -0.2
growth on ricinoleic acid YNB Glucose -0.6 N.D. N.D. -0.6 +0.4
growth on oleic acid YNB Glucose -0.2 N.D. N.D. -0.6 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. N.D. -0.5 -0.4
auxotrophy YNB Glucose -0.3 N.D. N.D. -0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.6 N.D. N.D. -0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.5 N.D. N.D. -0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. N.D. -0.4 -0.3
growth on oleic acid YNB Oleic Acid -0.2 N.D. N.D. +0.0 -0.1
auxotrophy Dropout Complete -0.6 N.D. N.D. +0.1 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. N.D. -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.5 N.D. N.D. -0.4 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. N.D. -0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 N.D. N.D. +0.1 -0.0
growth on ypd YPD +0.8 N.D. N.D. +0.1 +0.2
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