Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16475 and 16476 are separated by 337 nucleotides16476 and 16477 are separated by 591 nucleotides16477 and 16478 are separated by 547 nucleotides16478 and 16479 are separated by 193 nucleotides mRNA_8107: 16475 - K01937 pyrG, CTPS CTP synthase, at 427,104 to 429,524 16475 mRNA_8108: 16476 - K17637 EXOC2, SEC5 exocyst complex component 2, at 429,862 to 433,885 16476 mRNA_8109: 16477 - K00679 E2.3.1.158 phospholipid-diacylglycerol acyltransferase, at 434,477 to 437,050 16477 mRNA_8110: 16478 - HMMPfam-G protein-coupled glucose receptor regulating Gpa2-PF11710,SUPERFAMILY--SSF81321, at 437,598 to 439,156 16478 mRNA_8111: 16479 - HMMPfam-CTD kinase subunit gamma CTK3-PF12243,HMMPfam-CTD kinase subunit gamma CTK3 C-terminus-PF12350,ProSiteProfiles-CID domain profile.-PS51391, at 439,350 to 440,597 16479
Group Condition mRNA_8107 mRNA_8108 mRNA_8109 mRNA_8110 mRNA_8111
16475 16476 16477 16478 16479
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -1.0 -0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.6 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.8 -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.5 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.4 -0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.8 +0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.7 +0.2 N.D.
growth on oleic acid YNB Glucose N.D. N.D. -0.6 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.5 +0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.4 +0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.3 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.1 -0.1 N.D.
auxotrophy YNB Glucose N.D. N.D. -0.3 +0.1 N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.6 +0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.1 +0.1 N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.0 -0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.1 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.1 -0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.1 +0.2 N.D.
growth on ypd YPD N.D. N.D. +0.1 +0.2 N.D.
auxotrophy Dropout Complete N.D. N.D. +0.1 +0.3 N.D.
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