Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16477 and 16478 are separated by 547 nucleotides16478 and 16479 are separated by 193 nucleotides16479 and 16480 are separated by 134 nucleotides16480 and 16481 are separated by 521 nucleotides mRNA_8109: 16477 - K00679 E2.3.1.158 phospholipid-diacylglycerol acyltransferase, at 434,477 to 437,050 16477 mRNA_8110: 16478 - HMMPfam-G protein-coupled glucose receptor regulating Gpa2-PF11710,SUPERFAMILY--SSF81321, at 437,598 to 439,156 16478 mRNA_8111: 16479 - HMMPfam-CTD kinase subunit gamma CTK3-PF12243,HMMPfam-CTD kinase subunit gamma CTK3 C-terminus-PF12350,ProSiteProfiles-CID domain profile.-PS51391, at 439,350 to 440,597 16479 mRNA_8112: 16480 - K14772 UTP20 U3 small nucleolar RNA-associated protein 20, at 440,732 to 449,683 16480 mRNA_8113: 16481 - HMMPfam-Thioesterase-like superfamily-PF13279,SUPERFAMILY--SSF54637, at 450,205 to 451,053 16481
Group Condition mRNA_8109 mRNA_8110 mRNA_8111 mRNA_8112 mRNA_8113
16477 16478 16479 16480 16481
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 -0.4 N.D. N.D. +0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -0.4 N.D. N.D. -0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 -0.3 N.D. N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.8 +0.2 N.D. N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 +0.1 N.D. N.D. -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 -0.3 N.D. N.D. -0.1
growth on ricinoleic acid YNB Glucose -0.6 +0.4 N.D. N.D. -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.8 -0.2 N.D. N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.5 +0.0 N.D. N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.4 +0.0 N.D. N.D. -0.2
growth on oleic acid YNB Glucose -0.6 +0.1 N.D. N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.1 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.1 N.D. N.D. +0.3
growth on oleic acid YNB Oleic Acid +0.0 -0.1 N.D. N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.1 N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine -0.7 +0.2 N.D. N.D. +0.6
growth on ypd YPD +0.1 +0.2 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.0 N.D. N.D. +0.2
auxotrophy YNB Glucose -0.3 +0.1 N.D. N.D. +0.5
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.2 N.D. N.D. +0.4
auxotrophy Dropout Complete +0.1 +0.3 N.D. N.D. +0.5
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