Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16478 and 16479 are separated by 193 nucleotides16479 and 16480 are separated by 134 nucleotides16480 and 16481 are separated by 521 nucleotides16481 and 16482 are separated by 355 nucleotides mRNA_8110: 16478 - HMMPfam-G protein-coupled glucose receptor regulating Gpa2-PF11710,SUPERFAMILY--SSF81321, at 437,598 to 439,156 16478 mRNA_8111: 16479 - HMMPfam-CTD kinase subunit gamma CTK3-PF12243,HMMPfam-CTD kinase subunit gamma CTK3 C-terminus-PF12350,ProSiteProfiles-CID domain profile.-PS51391, at 439,350 to 440,597 16479 mRNA_8112: 16480 - K14772 UTP20 U3 small nucleolar RNA-associated protein 20, at 440,732 to 449,683 16480 mRNA_8113: 16481 - HMMPfam-Thioesterase-like superfamily-PF13279,SUPERFAMILY--SSF54637, at 450,205 to 451,053 16481 mRNA_8114: 16482 - K09648 IMP2 mitochondrial inner membrane protease subunit 2, at 451,409 to 451,898 16482
Group Condition mRNA_8110 mRNA_8111 mRNA_8112 mRNA_8113 mRNA_8114
16478 16479 16480 16481 16482
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. N.D. -0.5 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. N.D. -0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. N.D. -0.5 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. N.D. -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. N.D. -0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. -0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. N.D. -0.2 N.D.
growth on ricinoleic acid YNB Glucose +0.4 N.D. N.D. -0.5 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. N.D. -0.2 N.D.
growth on oleic acid YNB Oleic Acid -0.1 N.D. N.D. +0.1 N.D.
growth on oleic acid YNB Glucose +0.1 N.D. N.D. -0.0 N.D.
growth on ypd YPD +0.2 N.D. N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. N.D. +0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. N.D. +0.4 N.D.
auxotrophy YNB Glucose +0.1 N.D. N.D. +0.5 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. N.D. +0.6 N.D.
auxotrophy Dropout Complete +0.3 N.D. N.D. +0.5 N.D.
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