Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16481 and 16482 are separated by 355 nucleotides16482 and 16483 are separated by 410 nucleotides16483 and 16484 are separated by 336 nucleotides16484 and 16485 are separated by 389 nucleotides mRNA_8113: 16481 - HMMPfam-Thioesterase-like superfamily-PF13279,SUPERFAMILY--SSF54637, at 450,205 to 451,053 16481 mRNA_8114: 16482 - K09648 IMP2 mitochondrial inner membrane protease subunit 2, at 451,409 to 451,898 16482 mRNA_8115: 16483 - K03868 RBX1, ROC1 RING-box protein 1, at 452,309 to 453,227 16483 mRNA_8116: 16484 - K00949 thiN, TPK1, THI80 thiamine pyrophosphokinase, at 453,564 to 454,690 16484 mRNA_8117: 16485 - K06041 kdsD, kpsF arabinose-5-phosphate isomerase, at 455,080 to 456,482 16485
Group Condition mRNA_8113 mRNA_8114 mRNA_8115 mRNA_8116 mRNA_8117
16481 16482 16483 16484 16485
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 N.D. N.D. -5.0 +0.9
growth on ricinoleic acid YNB Glucose -0.5 N.D. N.D. -3.7 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. N.D. -3.7 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. N.D. -3.0 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.4 N.D. N.D. -4.3 +0.8
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. N.D. -2.5 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.6 N.D. N.D. -3.5 +0.6
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. N.D. -1.5 +0.1
growth on oleic acid YNB Glucose -0.0 N.D. N.D. -2.0 +0.4
auxotrophy YNB Glucose +0.5 N.D. N.D. -2.8 +0.7
auxotrophy Dropout Complete +0.5 N.D. N.D. -2.7 +0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. N.D. -1.2 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. N.D. -1.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. N.D. -0.6 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. N.D. -0.5 +0.0
growth on oleic acid YNB Oleic Acid +0.1 N.D. N.D. -0.9 +0.6
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. N.D. -0.0 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. N.D. -0.0 +0.3
growth on ypd YPD -0.2 N.D. N.D. +0.4 +0.0
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. N.D. +0.8 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. N.D. +1.4 +0.3
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