Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16482 and 16483 are separated by 410 nucleotides16483 and 16484 are separated by 336 nucleotides16484 and 16485 are separated by 389 nucleotides16485 and 16486 are separated by 426 nucleotides mRNA_8114: 16482 - K09648 IMP2 mitochondrial inner membrane protease subunit 2, at 451,409 to 451,898 16482 mRNA_8115: 16483 - K03868 RBX1, ROC1 RING-box protein 1, at 452,309 to 453,227 16483 mRNA_8116: 16484 - K00949 thiN, TPK1, THI80 thiamine pyrophosphokinase, at 453,564 to 454,690 16484 mRNA_8117: 16485 - K06041 kdsD, kpsF arabinose-5-phosphate isomerase, at 455,080 to 456,482 16485 mRNA_8118: 16486 - Hypothetical Protein, at 456,909 to 458,986 16486
Group Condition mRNA_8114 mRNA_8115 mRNA_8116 mRNA_8117 mRNA_8118
16482 16483 16484 16485 16486
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -5.0 +0.9 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -3.7 +0.3 -0.5
growth on ricinoleic acid YNB Glucose N.D. N.D. -3.7 +0.3 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -4.3 +0.8 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -3.0 -0.2 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -3.5 +0.6 +0.1
auxotrophy YNB Glucose N.D. N.D. -2.8 +0.7 -0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -2.5 -0.0 -0.1
auxotrophy Dropout Complete N.D. N.D. -2.7 +0.9 -0.4
growth on oleic acid YNB Glucose N.D. N.D. -2.0 +0.4 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -1.1 +0.1 +0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.9 +0.6 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -1.5 +0.1 +0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.5 +0.0 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -1.2 +0.3 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.6 +0.2 +0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.0 +0.3 -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.0 +0.2 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +1.4 +0.3 -1.6
growth on ypd YPD N.D. N.D. +0.4 +0.0 +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.8 +0.2 +0.4
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