Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16483 and 16484 are separated by 336 nucleotides16484 and 16485 are separated by 389 nucleotides16485 and 16486 are separated by 426 nucleotides16486 and 16487 are separated by 295 nucleotides mRNA_8115: 16483 - K03868 RBX1, ROC1 RING-box protein 1, at 452,309 to 453,227 16483 mRNA_8116: 16484 - K00949 thiN, TPK1, THI80 thiamine pyrophosphokinase, at 453,564 to 454,690 16484 mRNA_8117: 16485 - K06041 kdsD, kpsF arabinose-5-phosphate isomerase, at 455,080 to 456,482 16485 mRNA_8118: 16486 - Hypothetical Protein, at 456,909 to 458,986 16486 mRNA_8119: 16487 - Hypothetical Protein, at 459,282 to 461,489 16487
Group Condition mRNA_8115 mRNA_8116 mRNA_8117 mRNA_8118 mRNA_8119
16483 16484 16485 16486 16487
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -3.0 -0.2 +0.1 -2.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -3.7 +0.3 -0.5 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -5.0 +0.9 -0.0 +0.7
auxotrophy YNB Glucose 75mM Methionine N.D. -4.3 +0.8 -0.1 +0.5
growth on ricinoleic acid YNB Glucose N.D. -3.7 +0.3 -0.3 +0.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. -2.5 -0.0 -0.1 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -3.5 +0.6 +0.1 +0.4
auxotrophy YNB Glucose N.D. -2.8 +0.7 -0.4 +0.6
auxotrophy Dropout Complete N.D. -2.7 +0.9 -0.4 +0.2
growth on oleic acid YNB Glucose N.D. -2.0 +0.4 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -1.1 +0.1 +0.2 -0.8
facs on lipid accumulation Sorted High BODIPY Gate N.D. -1.5 +0.1 +0.8 -0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.5 +0.0 -0.0 -0.6
growth on oleic acid YNB Oleic Acid N.D. -0.9 +0.6 -0.3 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.6 +0.2 +0.0 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -1.2 +0.3 +0.5 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 +0.3 -0.3 -0.2
growth on ypd YPD N.D. +0.4 +0.0 +0.3 -0.9
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 +0.2 -0.2 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +1.4 +0.3 -1.6 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.8 +0.2 +0.4 -0.2
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