Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16501 and 16502 are separated by 244 nucleotides16502 and 16503 are separated by 136 nucleotides16503 and 16504 are separated by 211 nucleotides16504 and 16505 are separated by 737 nucleotides mRNA_8133: 16501 - HMMPfam-Senescence-associated protein-PF06911, at 497,849 to 499,471 16501 mRNA_8134: 16502 - K17891 ERV2 FAD-linked sulfhydryl oxidase, at 499,716 to 500,735 16502 mRNA_8135: 16503 - K00804 GGPS1 geranylgeranyl diphosphate synthase, type III, at 500,872 to 502,376 16503 mRNA_8136: 16504 - KOG0157 Cytochrome P450 CYP4/CYP19/CYP26 subfamilies, at 502,588 to 504,412 16504 mRNA_8137: 16505 - K00586 DPH5 diphthine methyl ester synthase, at 505,150 to 506,230 16505
Group Condition mRNA_8133 mRNA_8134 mRNA_8135 mRNA_8136 mRNA_8137
16501 16502 16503 16504 16505
facs on lipid accumulation Sorted Low BODIPY Gate +0.7 +0.1 -3.6 -0.6 -3.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.3 -3.4 +0.3 -2.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 -0.3 -0.6 -0.4 -3.8
growth on oleic acid YNB Glucose -0.7 -0.3 -1.4 -0.5 -1.7
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 -0.3 -1.5 -0.2 -1.9
growth on ricinoleic acid YNB Glucose +0.0 -0.1 -0.8 -0.4 -2.4
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.1 -2.9 +0.1 -1.1
auxotrophy Dropout Complete +0.3 +0.2 -1.3 +0.1 -2.5
auxotrophy YNB Glucose +0.4 +0.4 -1.7 +0.5 -2.2
growth on oleic acid YNB Oleic Acid -0.3 +0.1 -1.0 -0.3 -1.1
growth on ypd YPD -0.0 +0.0 -1.7 +0.5 -0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.5 -1.9 +0.2 -0.7
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.5 -1.1 +0.3 -1.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.3 -0.6 -0.3 +0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 -0.0 -0.6 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 +0.2 +0.2 -0.1 +1.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.4 +0.1 -0.2 +1.5
ypd buoyancy 40 hrs Low Buoyancy +1.2 +0.3 +0.9 +0.2 -0.1
ypd buoyancy 40 hrs Median Buoyancy +1.8 +0.2 +0.2 +0.3 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +1.4 -0.5 +1.6 +0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy +1.5 +0.3 +0.6 +0.0 +1.9
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