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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
16503 and 16504 are separated by 211 nucleotides
16504 and 16505 are separated by 737 nucleotides
16505 and 16506 are separated by 180 nucleotides
16506 and 16507 are separated by 1632 nucleotides
mRNA_8135: 16503 - K00804 GGPS1 geranylgeranyl diphosphate synthase, type III, at 500,872 to 502,376
16503
mRNA_8136: 16504 - KOG0157 Cytochrome P450 CYP4/CYP19/CYP26 subfamilies, at 502,588 to 504,412
16504
mRNA_8137: 16505 - K00586 DPH5 diphthine methyl ester synthase, at 505,150 to 506,230
16505
mRNA_8138: 16506 - Hypothetical Protein, at 506,411 to 507,790
16506
mRNA_8139: 16507 - K01139 spoT, HDDC3 guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 509,423 to 510,293
16507
Group
Condition
mRNA
_8135
mRNA
_8136
mRNA
_8137
mRNA
_8138
mRNA
_8139
16503
16504
16505
16506
16507
facs on lipid accumulation
Sorted Low BODIPY Gate
-3.6
-0.6
-3.1
-0.7
-0.2
facs on lipid accumulation
Sorted High BODIPY Gate
-3.4
+0.3
-2.4
-0.2
-0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.6
-0.4
-3.8
-0.3
-0.0
growth on oleic acid
YNB Glucose
-1.4
-0.5
-1.7
+0.1
-0.3
growth on ricinoleic acid
YNB Glucose
-0.8
-0.4
-2.4
+0.2
-0.2
auxotrophy
Dropout Complete
-1.3
+0.1
-2.5
+0.3
-0.2
facs on lipid accumulation
Sorted No BODIPY Gate
-2.9
+0.1
-1.1
-0.0
+0.3
auxotrophy
YNB Glucose
-1.7
+0.5
-2.2
+0.2
-0.2
growth on ricinoleic acid
YNB Ricinoleic Acid
-1.5
-0.2
-1.9
-0.1
+0.6
growth on oleic acid
YNB Oleic Acid
-1.0
-0.3
-1.1
+0.0
+0.4
growth on ypd
YPD
-1.7
+0.5
-0.4
+0.2
-0.5
auxotrophy
YNB Glucose 75mM Methionine
-1.9
+0.2
-0.7
+0.9
+0.1
auxotrophy
YNB Glucose 75mM Arginine
-1.1
+0.3
-1.4
+0.5
+0.3
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.6
+0.1
+0.0
-1.0
+0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.6
-0.3
+0.8
-0.2
-0.2
ypd buoyancy
40 hrs Median Buoyancy
+0.2
+0.3
+0.1
-0.1
-0.5
ypd buoyancy
40 hrs Low Buoyancy
+0.9
+0.2
-0.1
-0.2
-0.7
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.1
-0.2
+1.5
-0.3
-0.3
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.2
-0.1
+1.2
-0.4
+0.1
ypd buoyancy
40 hrs No Buoyancy Separation
+1.6
+0.2
+0.2
+0.7
-1.8
ypd buoyancy
40 hrs High Buoyancy
+0.6
+0.0
+1.9
-0.4
-0.7
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