Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16512 and 16513 are separated by 134 nucleotides16513 and 16514 are separated by 208 nucleotides16514 and 16515 are separated by 281 nucleotides16515 and 16516 are separated by 321 nucleotides mRNA_8144: 16512 - HMMPfam-Domain of unknown function (DUF3506)-PF12014,HMMPfam-F-box-like-PF12937,ProSiteProfiles-F-box domain profile.-PS50181,SMART-A Receptor for Ubiquitination Targets-SM00256,SUPERFAMILY--SSF81383, at 515,756 to 518,130 16512 mRNA_8145: 16513 - K15304 RANBP3 Ran-binding protein 3, at 518,265 to 522,947 16513 mRNA_8146: 16514 - HMMPfam-PH domain-PF00169,ProSiteProfiles-PH domain profile.-PS50003,SMART-Pleckstrin homology domain.-SM00233,SUPERFAMILY--SSF50729,SUPERFAMILY--SSF55753, at 523,156 to 525,295 16514 mRNA_8147: 16515 - HMMPfam-Enoyl-CoA hydratase/isomerase family-PF00378,SUPERFAMILY--SSF52096, at 525,577 to 526,736 16515 mRNA_8148: 16516 - HMMPfam-START domain-PF01852,ProSiteProfiles-START domain profile.-PS50848,SUPERFAMILY--SSF55961, at 527,058 to 528,382 16516
Group Condition mRNA_8144 mRNA_8145 mRNA_8146 mRNA_8147 mRNA_8148
16512 16513 16514 16515 16516
growth on ricinoleic acid YNB Glucose +0.1 +0.0 -0.8 +0.2 -1.0
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.1 -0.8 -0.5 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.3 -0.9 +0.4 -0.6
auxotrophy YNB Glucose 75mM Arginine -0.4 -0.1 -0.9 +0.1 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.1 +0.4 +0.1 -1.4
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.1 -0.4 -0.2 -0.3
auxotrophy YNB Glucose -0.6 -0.1 -0.7 -0.0 +0.8
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 +0.3 +0.0 -0.1 -0.5
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.1 +0.0 -0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.5 -0.1 +0.2 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -0.2 -0.6 -0.2 +0.8
growth on oleic acid YNB Glucose -0.4 +0.1 -0.1 -0.2 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +0.2 +0.6 -0.4 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.1 +0.7 -0.3 -0.0
growth on oleic acid YNB Oleic Acid +0.0 +0.1 -0.6 -0.1 +0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.1 +0.3 -0.2 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -0.0 -0.3 +0.1 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +0.2 +0.6 -0.1 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.0 -0.1 -0.3 +1.2
growth on ypd YPD -0.1 -0.2 +0.7 +0.3 -0.3
auxotrophy Dropout Complete -0.3 -0.1 +0.6 -0.1 +0.9
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