Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16513 and 16514 are separated by 208 nucleotides16514 and 16515 are separated by 281 nucleotides16515 and 16516 are separated by 321 nucleotides16516 and 16517 are separated by 547 nucleotides mRNA_8145: 16513 - K15304 RANBP3 Ran-binding protein 3, at 518,265 to 522,947 16513 mRNA_8146: 16514 - HMMPfam-PH domain-PF00169,ProSiteProfiles-PH domain profile.-PS50003,SMART-Pleckstrin homology domain.-SM00233,SUPERFAMILY--SSF50729,SUPERFAMILY--SSF55753, at 523,156 to 525,295 16514 mRNA_8147: 16515 - HMMPfam-Enoyl-CoA hydratase/isomerase family-PF00378,SUPERFAMILY--SSF52096, at 525,577 to 526,736 16515 mRNA_8148: 16516 - HMMPfam-START domain-PF01852,ProSiteProfiles-START domain profile.-PS50848,SUPERFAMILY--SSF55961, at 527,058 to 528,382 16516 mRNA_8149: 16517 - KOG1198 Zinc-binding oxidoreductase, at 528,930 to 530,418 16517
Group Condition mRNA_8145 mRNA_8146 mRNA_8147 mRNA_8148 mRNA_8149
16513 16514 16515 16516 16517
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.9 +0.4 -0.6 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.4 +0.1 -1.4 -0.6
growth on ricinoleic acid YNB Glucose +0.0 -0.8 +0.2 -1.0 +0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.8 -0.5 -0.4 +0.6
ypd buoyancy 40 hrs Low Buoyancy -0.5 -0.1 +0.2 +0.0 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.4 -0.2 -0.3 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.6 -0.2 +0.8 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.6 -0.4 -0.2 -0.8
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.0 -0.1 -0.1 -0.3
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.9 +0.1 +0.3 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.6 -0.1 -0.3 -0.7
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.3 +0.1 +0.3 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.3 -0.2 +0.1 -0.2
auxotrophy YNB Glucose -0.1 -0.7 -0.0 +0.8 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.0 -0.1 -0.5 +0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.7 -0.3 -0.0 +0.1
growth on oleic acid YNB Glucose +0.1 -0.1 -0.2 +0.3 +0.6
growth on ypd YPD -0.2 +0.7 +0.3 -0.3 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.1 -0.3 +1.2 +0.1
growth on oleic acid YNB Oleic Acid +0.1 -0.6 -0.1 +0.9 +0.8
auxotrophy Dropout Complete -0.1 +0.6 -0.1 +0.9 +0.3
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