Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16514 and 16515 are separated by 281 nucleotides16515 and 16516 are separated by 321 nucleotides16516 and 16517 are separated by 547 nucleotides16517 and 16518 are separated by 160 nucleotides mRNA_8146: 16514 - HMMPfam-PH domain-PF00169,ProSiteProfiles-PH domain profile.-PS50003,SMART-Pleckstrin homology domain.-SM00233,SUPERFAMILY--SSF50729,SUPERFAMILY--SSF55753, at 523,156 to 525,295 16514 mRNA_8147: 16515 - HMMPfam-Enoyl-CoA hydratase/isomerase family-PF00378,SUPERFAMILY--SSF52096, at 525,577 to 526,736 16515 mRNA_8148: 16516 - HMMPfam-START domain-PF01852,ProSiteProfiles-START domain profile.-PS50848,SUPERFAMILY--SSF55961, at 527,058 to 528,382 16516 mRNA_8149: 16517 - KOG1198 Zinc-binding oxidoreductase, at 528,930 to 530,418 16517 mRNA_8150: 16518 - K11135 PINX1 Pin2-interacting protein X1, at 530,579 to 532,165 16518
Group Condition mRNA_8146 mRNA_8147 mRNA_8148 mRNA_8149 mRNA_8150
16514 16515 16516 16517 16518
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 +0.1 -1.4 -0.6 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.9 +0.4 -0.6 -0.0 N.D.
growth on ricinoleic acid YNB Glucose -0.8 +0.2 -1.0 +0.5 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.8 -0.5 -0.4 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 -0.4 -0.2 -0.8 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.4 -0.2 -0.3 +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.1 -0.1 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 -0.1 -0.3 -0.7 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 -0.2 +0.8 -0.4 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.9 +0.1 +0.3 +0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.2 +0.0 -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.1 +0.3 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.2 +0.1 -0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.1 -0.5 +0.7 N.D.
auxotrophy YNB Glucose -0.7 -0.0 +0.8 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.7 -0.3 -0.0 +0.1 N.D.
growth on oleic acid YNB Glucose -0.1 -0.2 +0.3 +0.6 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.3 +1.2 +0.1 N.D.
growth on ypd YPD +0.7 +0.3 -0.3 +0.2 N.D.
growth on oleic acid YNB Oleic Acid -0.6 -0.1 +0.9 +0.8 N.D.
auxotrophy Dropout Complete +0.6 -0.1 +0.9 +0.3 N.D.
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