Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16524 and 16525 are separated by 887 nucleotides16525 and 16526 are separated by 192 nucleotides16526 and 16527 are separated by 185 nucleotides16527 and 16528 are separated by 134 nucleotides mRNA_8156: 16524 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 545,872 to 548,769 16524 mRNA_8157: 16525 - ProSiteProfiles-DOMON domain profile.-PS50836,ProSiteProfiles-Cytochrome b561 domain profile.-PS50939,SMART-Cytochrome b-561 / ferric reductase transmembrane domain.-SM00665,SUPERFAMILY--SSF49344, at 549,657 to 551,853 16525 mRNA_8158: 16526 - K14004 SEC13 protein transport protein SEC13, at 552,046 to 553,498 16526 mRNA_8159: 16527 - K17607 TIPRL, TIP41 type 2A phosphatase activator TIP41, at 553,684 to 555,009 16527 mRNA_8160: 16528 - K01873 VARS, valS valyl-tRNA synthetase, at 555,144 to 559,347 16528
Group Condition mRNA_8156 mRNA_8157 mRNA_8158 mRNA_8159 mRNA_8160
16524 16525 16526 16527 16528
facs on lipid accumulation Sorted Low BODIPY Gate -2.2 -0.5 N.D. -1.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.3 -0.2 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.1 +0.2 N.D. -0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.7 +0.1 N.D. -0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.1 N.D. -0.3 N.D.
growth on ricinoleic acid YNB Glucose -0.9 -0.0 N.D. +0.6 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.2 N.D. -0.3 N.D.
auxotrophy YNB Glucose -1.3 -0.1 N.D. +1.2 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.7 -0.1 N.D. +0.7 N.D.
auxotrophy YNB Glucose 75mM Methionine -1.0 +0.0 N.D. +0.9 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.0 N.D. +0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 +0.1 N.D. +0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.0 N.D. -0.0 N.D.
growth on oleic acid YNB Glucose -0.7 +0.0 N.D. +0.9 N.D.
auxotrophy Dropout Complete -0.8 -0.0 N.D. +1.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 +0.1 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.0 N.D. +0.3 N.D.
growth on ypd YPD +0.0 +0.1 N.D. +0.5 N.D.
growth on oleic acid YNB Oleic Acid -0.3 +0.0 N.D. +1.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.1 N.D. +1.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.8 +0.2 N.D. +0.3 N.D.
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