Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16525 and 16526 are separated by 192 nucleotides16526 and 16527 are separated by 185 nucleotides16527 and 16528 are separated by 134 nucleotides16528 and 16529 are separated by 179 nucleotides mRNA_8157: 16525 - ProSiteProfiles-DOMON domain profile.-PS50836,ProSiteProfiles-Cytochrome b561 domain profile.-PS50939,SMART-Cytochrome b-561 / ferric reductase transmembrane domain.-SM00665,SUPERFAMILY--SSF49344, at 549,657 to 551,853 16525 mRNA_8158: 16526 - K14004 SEC13 protein transport protein SEC13, at 552,046 to 553,498 16526 mRNA_8159: 16527 - K17607 TIPRL, TIP41 type 2A phosphatase activator TIP41, at 553,684 to 555,009 16527 mRNA_8160: 16528 - K01873 VARS, valS valyl-tRNA synthetase, at 555,144 to 559,347 16528 mRNA_8161: 16529 - BLAST Actin cytoskeleton-regulatory complex protein pan1 [R..., at 559,527 to 560,747 16529
Group Condition mRNA_8157 mRNA_8158 mRNA_8159 mRNA_8160 mRNA_8161
16525 16526 16527 16528 16529
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 N.D. -1.3 N.D. +0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. -0.3 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. -0.2 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. -0.1 N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. -0.0 N.D. -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. -0.3 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. -0.1 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.1 N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. +0.3 N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. +0.3 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. +0.3 N.D. +0.0
growth on ricinoleic acid YNB Glucose -0.0 N.D. +0.6 N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. +0.4 N.D. +0.2
growth on ypd YPD +0.1 N.D. +0.5 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. +0.7 N.D. +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. +1.0 N.D. -0.0
growth on oleic acid YNB Glucose +0.0 N.D. +0.9 N.D. +0.2
growth on oleic acid YNB Oleic Acid +0.0 N.D. +1.1 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. +0.9 N.D. +0.5
auxotrophy YNB Glucose -0.1 N.D. +1.2 N.D. +0.4
auxotrophy Dropout Complete -0.0 N.D. +1.1 N.D. +0.8
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