Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16527 and 16528 are separated by 134 nucleotides16528 and 16529 are separated by 179 nucleotides16529 and 16530 are separated by 166 nucleotides16530 and 16531 are separated by 131 nucleotides mRNA_8159: 16527 - K17607 TIPRL, TIP41 type 2A phosphatase activator TIP41, at 553,684 to 555,009 16527 mRNA_8160: 16528 - K01873 VARS, valS valyl-tRNA synthetase, at 555,144 to 559,347 16528 mRNA_8161: 16529 - BLAST Actin cytoskeleton-regulatory complex protein pan1 [R..., at 559,527 to 560,747 16529 mRNA_8162: 16530 - K01288 KEX1 carboxypeptidase D, at 560,914 to 563,494 16530 mRNA_8163: 16531 - HMMPfam-Domain of unknown function (DUF4505)-PF14956, at 563,626 to 564,400 16531
Group Condition mRNA_8159 mRNA_8160 mRNA_8161 mRNA_8162 mRNA_8163
16527 16528 16529 16530 16531
facs on lipid accumulation Sorted Low BODIPY Gate -1.3 N.D. +0.4 -0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. -0.2 +0.3 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. +0.1 -0.4 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. -0.3 -0.3 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. -0.2 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. -0.0 -0.2 +0.3
growth on ricinoleic acid YNB Glucose +0.6 N.D. +0.0 -0.1 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. +0.2 +0.0 -0.6
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. +0.1 -0.0 +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. +0.0 -0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. +0.1 -0.1 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.3 N.D. +0.0 +0.0 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. +0.2 -0.0 +0.5
auxotrophy YNB Glucose 75mM Arginine +0.7 N.D. +0.4 -0.3 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +1.0 N.D. -0.0 +0.0 -0.5
growth on ypd YPD +0.5 N.D. +0.2 -0.0 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.9 N.D. +0.5 -0.3 -0.1
auxotrophy YNB Glucose +1.2 N.D. +0.4 -0.3 -0.0
growth on oleic acid YNB Glucose +0.9 N.D. +0.2 -0.0 +0.2
growth on oleic acid YNB Oleic Acid +1.1 N.D. +0.2 +0.1 +0.1
auxotrophy Dropout Complete +1.1 N.D. +0.8 -0.1 +0.2
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