Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16536 and 16537 are separated by 839 nucleotides16537 and 16538 are separated by 899 nucleotides16538 and 16539 are separated by 125 nucleotides16539 and 16540 are separated by 1100 nucleotides mRNA_8168: 16536 - BLAST PQ-loop-domain-containing protein [Dichomitus squ..., at 580,110 to 581,600 16536 mRNA_8169: 16537 - HMMPfam-Anaphase-promoting complex, cyclosome, subunit 4-PF12896, at 582,440 to 584,866 16537 mRNA_8170: 16538 - BLAST PQ-loop-domain-containing protein [Calocera cornea HH..., at 585,766 to 587,530 16538 mRNA_8171: 16539 - HMMPfam-DnaJ domain-PF00226,ProSiteProfiles-dnaJ domain profile.-PS50076,SMART-DnaJ molecular chaperone homology domain-SM00271,SUPERFAMILY--SSF46565, at 587,656 to 589,364 16539 mRNA_8172: 16540 - K17592 SACS sacsin, at 590,465 to 596,519 16540
Group Condition mRNA_8168 mRNA_8169 mRNA_8170 mRNA_8171 mRNA_8172
16536 16537 16538 16539 16540
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. +0.2 -0.0 -0.9
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. +0.0 -0.0 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. -0.1 -0.4 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. -0.1 -0.1 +0.0
growth on ricinoleic acid YNB Glucose -0.1 N.D. +0.5 -0.7 +0.2
growth on oleic acid YNB Glucose +0.1 N.D. +0.0 -0.2 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. -0.0 -0.1 +0.1
auxotrophy YNB Glucose -0.1 N.D. +0.2 +0.0 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. +0.3 -0.2 +0.1
growth on oleic acid YNB Oleic Acid -0.0 N.D. +0.0 +0.4 -0.1
growth on ypd YPD +0.3 N.D. +0.0 +0.2 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 N.D. +0.5 -0.3 +0.3
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.2 +0.0 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.2 +0.0 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. +0.2 +0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. +0.2 +0.2 -0.2
auxotrophy Dropout Complete -0.1 N.D. +0.2 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. -0.4 +0.6 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. -0.2 +0.4 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +0.1 +0.3 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. -0.0 +0.8 +0.1
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