Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16537 and 16538 are separated by 899 nucleotides16538 and 16539 are separated by 125 nucleotides16539 and 16540 are separated by 1100 nucleotides16540 and 16541 are separated by 237 nucleotides mRNA_8169: 16537 - HMMPfam-Anaphase-promoting complex, cyclosome, subunit 4-PF12896, at 582,440 to 584,866 16537 mRNA_8170: 16538 - BLAST PQ-loop-domain-containing protein [Calocera cornea HH..., at 585,766 to 587,530 16538 mRNA_8171: 16539 - HMMPfam-DnaJ domain-PF00226,ProSiteProfiles-dnaJ domain profile.-PS50076,SMART-DnaJ molecular chaperone homology domain-SM00271,SUPERFAMILY--SSF46565, at 587,656 to 589,364 16539 mRNA_8172: 16540 - K17592 SACS sacsin, at 590,465 to 596,519 16540 mRNA_8173: 16541 - HMMPfam-Glutathione-dependent formaldehyde-activating enzyme-PF04828,SUPERFAMILY--SSF51316, at 596,757 to 597,514 16541
Group Condition mRNA_8169 mRNA_8170 mRNA_8171 mRNA_8172 mRNA_8173
16537 16538 16539 16540 16541
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 +0.2 -0.2 -3.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 -0.0 -0.9 -2.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.0 -0.0 -0.1 -1.4
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 -0.4 +0.1 -0.9
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 +0.0 +0.0 -1.4
growth on ypd YPD N.D. +0.0 +0.2 -0.2 -0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 -0.1 +0.0 -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 -0.1 +0.1 -0.4
growth on ricinoleic acid YNB Glucose N.D. +0.5 -0.7 +0.2 -0.2
auxotrophy YNB Glucose N.D. +0.2 +0.0 -0.0 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.5 -0.3 +0.3 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 +0.3 +0.2 -0.5
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 +0.1 +0.1 -0.3
growth on oleic acid YNB Glucose N.D. +0.0 -0.2 +0.0 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 -0.2 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.4 +0.6 +0.1 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 +0.0 +0.2 +0.1
growth on oleic acid YNB Oleic Acid N.D. +0.0 +0.4 -0.1 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 +0.4 +0.2 +0.2
auxotrophy Dropout Complete N.D. +0.2 +0.2 +0.3 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.0 +0.8 +0.1 +0.2
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