Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16538 and 16539 are separated by 125 nucleotides16539 and 16540 are separated by 1100 nucleotides16540 and 16541 are separated by 237 nucleotides16541 and 16542 are separated by 1358 nucleotides mRNA_8170: 16538 - BLAST PQ-loop-domain-containing protein [Calocera cornea HH..., at 585,766 to 587,530 16538 mRNA_8171: 16539 - HMMPfam-DnaJ domain-PF00226,ProSiteProfiles-dnaJ domain profile.-PS50076,SMART-DnaJ molecular chaperone homology domain-SM00271,SUPERFAMILY--SSF46565, at 587,656 to 589,364 16539 mRNA_8172: 16540 - K17592 SACS sacsin, at 590,465 to 596,519 16540 mRNA_8173: 16541 - HMMPfam-Glutathione-dependent formaldehyde-activating enzyme-PF04828,SUPERFAMILY--SSF51316, at 596,757 to 597,514 16541 mRNA_8174: 16542 - K00645 fabD [acyl-carrier-protein] S-malonyltransferase, at 598,873 to 600,672 16542
Group Condition mRNA_8170 mRNA_8171 mRNA_8172 mRNA_8173 mRNA_8174
16538 16539 16540 16541 16542
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.0 -0.9 -2.0 -2.4
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.2 -0.2 -3.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 -0.0 -0.1 -1.4 -0.9
growth on ypd YPD +0.0 +0.2 -0.2 -0.9 -0.7
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.2 +0.1 +0.1 -1.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 -0.3 +0.3 -0.3 -1.1
growth on ricinoleic acid YNB Glucose +0.5 -0.7 +0.2 -0.2 -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.4 +0.1 -0.9 +0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.1 +0.0 -0.3 +0.1
auxotrophy YNB Glucose +0.2 +0.0 -0.0 -0.1 -0.4
growth on oleic acid YNB Glucose +0.0 -0.2 +0.0 +0.4 -0.6
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.0 +0.0 -1.4 +0.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.3 +0.2 -0.5 -0.3
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.0 +0.2 +0.1 -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 +0.6 +0.1 +0.0 -0.4
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 +0.1 -0.3 -0.2
growth on oleic acid YNB Oleic Acid +0.0 +0.4 -0.1 +0.3 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.1 +0.1 -0.4 +0.9
facs on lipid accumulation Sorted High BODIPY Gate -0.0 +0.8 +0.1 +0.2 -0.5
auxotrophy Dropout Complete +0.2 +0.2 +0.3 +0.2 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.4 +0.2 +0.2 +0.2
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