Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16554 and 16555 are separated by 841 nucleotides16555 and 16556 are separated by 285 nucleotides16556 and 16557 are separated by 258 nucleotides16557 and 16558 are separated by 127 nucleotides mRNA_8186: 16554 - Hypothetical Protein, at 622,930 to 623,583 16554 mRNA_8187: 16555 - K10949 KDELR ER lumen protein retaining receptor, at 624,425 to 625,437 16555 mRNA_8188: 16556 - K05012 CLCN3_4_5 chloride channel 3/4/5, at 625,723 to 629,622 16556 mRNA_8189: 16557 - HMMPfam-alpha/beta hydrolase fold-PF07859,SUPERFAMILY--SSF53474, at 629,881 to 631,255 16557 mRNA_8190: 16558 - KOG2757 Mannose-6-phosphate isomerase, at 631,383 to 634,543 16558
Group Condition mRNA_8186 mRNA_8187 mRNA_8188 mRNA_8189 mRNA_8190
16554 16555 16556 16557 16558
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 N.D. -1.0 -0.3 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 N.D. +0.6 -0.7 +0.2
auxotrophy Dropout Complete +0.1 N.D. +0.6 -0.5 -0.5
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.2 -0.3 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 N.D. +0.5 -0.3 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.4 +0.0 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. +0.2 -0.1 -0.1
growth on oleic acid YNB Oleic Acid +0.0 N.D. +0.1 -0.1 +0.2
auxotrophy YNB Glucose +0.2 N.D. +0.3 -0.3 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 N.D. +0.9 -0.2 +0.0
growth on oleic acid YNB Glucose +0.1 N.D. +0.3 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. +0.5 -0.2 -0.1
growth on ricinoleic acid YNB Glucose +0.1 N.D. +0.8 -0.6 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. +0.3 +0.3 -0.1
growth on ypd YPD +0.3 N.D. +0.2 +0.0 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. +0.8 -0.3 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.5 N.D. -0.5 +0.6 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. +0.5 +0.4 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 N.D. +0.6 +0.7 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. +1.0 -0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. +0.4 +1.0 +0.1
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