Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16556 and 16557 are separated by 258 nucleotides16557 and 16558 are separated by 127 nucleotides16558 and 16559 are separated by 150 nucleotides16559 and 16560 are separated by 258 nucleotides mRNA_8188: 16556 - K05012 CLCN3_4_5 chloride channel 3/4/5, at 625,723 to 629,622 16556 mRNA_8189: 16557 - HMMPfam-alpha/beta hydrolase fold-PF07859,SUPERFAMILY--SSF53474, at 629,881 to 631,255 16557 mRNA_8190: 16558 - KOG2757 Mannose-6-phosphate isomerase, at 631,383 to 634,543 16558 mRNA_8191: 16559 - HMMPfam-F-box domain-PF00646,ProSiteProfiles-F-box domain profile.-PS50181,SUPERFAMILY--SSF81383, at 634,694 to 637,536 16559 mRNA_8192: 16560 - K12780 REC8 meiotic recombination protein REC8, fungi type, at 637,795 to 641,120 16560
Group Condition mRNA_8188 mRNA_8189 mRNA_8190 mRNA_8191 mRNA_8192
16556 16557 16558 16559 16560
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 -0.3 +0.1 +0.2 -0.0
auxotrophy Dropout Complete +0.6 -0.5 -0.5 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 -0.3 -0.6 +0.0 +0.0
auxotrophy YNB Glucose +0.3 -0.3 +0.0 -0.4 +0.1
growth on oleic acid YNB Oleic Acid +0.1 -0.1 +0.2 -0.3 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.3 -0.2 -0.0 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 -0.7 +0.2 +0.0 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.1 -0.1 -0.2 +0.2
growth on oleic acid YNB Glucose +0.3 -0.1 -0.1 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.0 -0.2 +0.0 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.5 +0.6 +0.2 -0.1 +0.0
growth on ypd YPD +0.2 +0.0 +0.1 -0.1 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 -0.2 -0.1 +0.2 +0.0
growth on ricinoleic acid YNB Glucose +0.8 -0.6 +0.1 +0.0 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.8 -0.3 +0.2 -0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.3 -0.1 +0.0 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.9 -0.2 +0.0 -0.4 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.5 +0.4 -0.1 -0.0 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.6 +0.7 -0.1 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.0 -0.1 -0.1 -0.0 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.4 +1.0 +0.1 +0.1 -0.1
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