Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16562 and 16563 are separated by 487 nucleotides16563 and 16564 are separated by 110 nucleotides16564 and 16565 are separated by 1377 nucleotides16565 and 16566 are separated by 226 nucleotides mRNA_8194: 16562 - K20794 NAA40, NAT4 N-alpha-acetyltransferase 40, at 644,404 to 645,092 16562 mRNA_8195: 16563 - Hypothetical Protein, at 645,580 to 648,404 16563 mRNA_8196: 16564 - K13501 TRP1 anthranilate synthase / indole-3-glycerol phosphate synthase / phosphoribosylanthranilate isomerase, at 648,515 to 650,860 16564 mRNA_8197: 16565 - KOG2922 Uncharacterized conserved protein, at 652,238 to 654,491 16565 mRNA_8198: 16566 - K03190 ureD, ureH urease accessory protein, at 654,718 to 655,775 16566
Group Condition mRNA_8194 mRNA_8195 mRNA_8196 mRNA_8197 mRNA_8198
16562 16563 16564 16565 16566
ypd buoyancy 40 hrs No Buoyancy Separation -1.7 +0.0 N.D. +0.1 -0.1
growth on oleic acid YNB Glucose -0.2 +0.1 N.D. +0.2 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 +0.2 N.D. -0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.1 N.D. -0.3 -0.0
growth on oleic acid YNB Oleic Acid -0.1 +0.1 N.D. +0.1 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.0 N.D. +0.2 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.4 -0.2 N.D. -0.4 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.0 N.D. +0.3 -0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.1 N.D. +0.0 +0.0
auxotrophy YNB Glucose +0.3 -0.3 N.D. +0.3 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 -0.1 N.D. +0.5 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -0.2 N.D. +0.8 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.1 N.D. +0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.4 +0.1 N.D. +0.2 -0.4
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.1 N.D. +0.2 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.2 N.D. -0.4 +0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 +0.1 N.D. +0.7 -0.3
growth on ypd YPD -0.2 +0.1 N.D. +0.2 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -0.1 N.D. +0.5 +0.2
auxotrophy Dropout Complete +0.6 +0.1 N.D. +0.3 -0.3
growth on ricinoleic acid YNB Glucose +0.2 +0.2 N.D. -0.1 +0.6
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