Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16568 and 16569 are separated by 151 nucleotides16569 and 16570 are separated by 321 nucleotides16570 and 16571 are separated by 441 nucleotides16571 and 16572 are separated by 710 nucleotides mRNA_8200: 16568 - KOG4635 Vacuolar import and degradation protein, at 658,183 to 660,127 16568 mRNA_8201: 16569 - KOG0065 Pleiotropic drug resistance proteins (PDR1-15), ABC superfamily, at 660,279 to 661,499 16569 mRNA_8202: 16570 - K03283 HSPA1_8 heat shock 70kDa protein 1/8, at 661,821 to 664,157 16570 mRNA_8203: 16571 - KOG3289 Uncharacterized conserved protein encoded by sequence overlapping the COX4 gene, at 664,599 to 665,368 16571 mRNA_8204: 16572 - KOG3289 Uncharacterized conserved protein encoded by sequence overlapping the COX4 gene, at 666,079 to 666,849 16572
Group Condition mRNA_8200 mRNA_8201 mRNA_8202 mRNA_8203 mRNA_8204
16568 16569 16570 16571 16572
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 -0.2 N.D. -0.0 -2.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.3 N.D. -0.3 -1.8
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.2 N.D. -0.1 -1.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.2 N.D. -0.1 -1.6
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 -0.1 N.D. -0.8 -0.7
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.5 N.D. -0.1 -0.8
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.1 N.D. -0.3 -0.8
growth on ricinoleic acid YNB Glucose +0.1 +0.3 N.D. +0.3 -1.6
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.1 N.D. -0.1 -0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.0 N.D. +0.2 -0.8
auxotrophy YNB Glucose +0.1 +0.1 N.D. -0.1 -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.4 N.D. -0.2 -0.9
growth on oleic acid YNB Glucose -0.2 +0.2 N.D. -0.3 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.1 N.D. -0.0 -0.7
growth on oleic acid YNB Oleic Acid +0.1 +0.1 N.D. -0.5 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 -0.1 N.D. +0.0 -0.2
auxotrophy Dropout Complete +0.0 +0.1 N.D. +0.1 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.1 N.D. -0.0 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.1 N.D. +0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.1 N.D. +0.2 +0.5
growth on ypd YPD +0.0 +0.1 N.D. +0.2 +1.3
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