Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16569 and 16570 are separated by 321 nucleotides16570 and 16571 are separated by 441 nucleotides16571 and 16572 are separated by 710 nucleotides16572 and 16573 are separated by 238 nucleotides mRNA_8201: 16569 - KOG0065 Pleiotropic drug resistance proteins (PDR1-15), ABC superfamily, at 660,279 to 661,499 16569 mRNA_8202: 16570 - K03283 HSPA1_8 heat shock 70kDa protein 1/8, at 661,821 to 664,157 16570 mRNA_8203: 16571 - KOG3289 Uncharacterized conserved protein encoded by sequence overlapping the COX4 gene, at 664,599 to 665,368 16571 mRNA_8204: 16572 - KOG3289 Uncharacterized conserved protein encoded by sequence overlapping the COX4 gene, at 666,079 to 666,849 16572 mRNA_8205: 16573 - K11339 MORF4L1, MRG15, EAF3 mortality factor 4-like protein 1, at 667,088 to 668,435 16573
Group Condition mRNA_8201 mRNA_8202 mRNA_8203 mRNA_8204 mRNA_8205
16569 16570 16571 16572 16573
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. -0.3 -1.8 -2.9
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. -0.1 -1.6 -2.8
facs on lipid accumulation Sorted No BODIPY Gate -0.5 N.D. -0.1 -0.8 -2.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. -0.8 -0.7 -0.8
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. -0.1 -0.9 -0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. -0.0 -2.3 +1.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. -0.1 -1.6 +0.9
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. -0.0 +0.1 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. -0.0 -0.7 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. -0.3 -0.8 +0.9
growth on ricinoleic acid YNB Glucose +0.3 N.D. +0.3 -1.6 +1.0
auxotrophy Dropout Complete +0.1 N.D. +0.1 -0.4 +0.4
auxotrophy YNB Glucose +0.1 N.D. -0.1 -0.7 +0.9
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. -0.2 -0.9 +0.9
growth on oleic acid YNB Oleic Acid +0.1 N.D. -0.5 -0.2 +0.9
growth on ypd YPD +0.1 N.D. +0.2 +1.3 -1.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.1 -0.0 +0.4
growth on oleic acid YNB Glucose +0.2 N.D. -0.3 -0.3 +0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. +0.2 -0.8 +1.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.2 +0.5 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. +0.0 -0.2 +1.6
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