Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16578 and 16579 are separated by 1382 nucleotides16579 and 16580 are separated by 205 nucleotides16580 and 16581 are separated by 137 nucleotides16581 and 16582 are separated by 225 nucleotides mRNA_8210: 16578 - BLAST WetA [Penicillium camemberti], at 676,267 to 678,027 16578 mRNA_8211: 16579 - K02894 RP-L23e, RPL23 large subunit ribosomal protein L23e, at 679,410 to 680,572 16579 mRNA_8212: 16580 - K17878 NNT1 nicotinamide N-methyltransferase, at 680,778 to 681,996 16580 mRNA_8213: 16581 - KOG1590 Uncharacterized conserved protein, at 682,134 to 683,094 16581 mRNA_8214: 16582 - BLAST NADH-ubiquinone oxidoreductase 9.5 kDa subunit [R..., at 683,320 to 684,168 16582
Group Condition mRNA_8210 mRNA_8211 mRNA_8212 mRNA_8213 mRNA_8214
16578 16579 16580 16581 16582
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. -0.3 -1.4 -0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 N.D. -0.1 -2.2 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. +0.0 -1.9 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. +0.5 +1.5 -3.9
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. +0.0 -2.5 +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. -0.1 -0.5 -1.5
growth on oleic acid YNB Glucose +0.2 N.D. -0.5 -0.5 -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.5 N.D. +0.3 -1.9 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.6 N.D. +0.1 -0.9 -0.2
auxotrophy Dropout Complete +0.2 N.D. -0.3 +0.3 -0.5
growth on ricinoleic acid YNB Glucose -0.1 N.D. -0.0 -0.8 +0.7
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.2 -0.6 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. -0.2 +0.3 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. -0.0 -1.3 +1.1
auxotrophy YNB Glucose +0.4 N.D. -0.2 +0.7 -0.5
growth on ypd YPD +0.1 N.D. -0.7 +0.5 +0.5
ypd buoyancy 40 hrs Median Buoyancy -0.3 N.D. +0.5 +0.5 +0.0
growth on oleic acid YNB Oleic Acid +0.1 N.D. -0.2 +0.3 +0.6
auxotrophy YNB Glucose 75mM Arginine +0.5 N.D. -0.2 +0.5 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.7 N.D. -0.2 +1.5 -1.2
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. +0.3 +1.2 -0.2
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