Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16587 and 16588 are separated by 228 nucleotides16588 and 16589 are separated by 100 nucleotides16589 and 16590 are separated by 374 nucleotides16590 and 16591 are separated by 326 nucleotides mRNA_8219: 16587 - K15202 GTF3C5, TFC1 general transcription factor 3C polypeptide 5 (transcription factor C subunit 1), at 698,551 to 700,808 16587 mRNA_8220: 16588 - KOG3894 SNARE protein Syntaxin 18/UFE1, at 701,037 to 702,340 16588 mRNA_8221: 16589 - KOG3140 Predicted membrane protein, at 702,441 to 703,886 16589 mRNA_8222: 16590 - KOG3140 Predicted membrane protein, at 704,261 to 706,813 16590 mRNA_8223: 16591 - K00801 FDFT1 farnesyl-diphosphate farnesyltransferase, at 707,140 to 708,844 16591
Group Condition mRNA_8219 mRNA_8220 mRNA_8221 mRNA_8222 mRNA_8223
16587 16588 16589 16590 16591
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. -0.5 -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. -0.7 +0.0 N.D.
auxotrophy YNB Glucose -0.0 N.D. -0.9 +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. -1.0 +0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. -0.1 -0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.3 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 N.D. -0.3 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.7 N.D. -0.0 +0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. +0.1 -0.3 N.D.
growth on oleic acid YNB Oleic Acid +0.3 N.D. -0.6 +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.5 N.D. +0.4 -0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. -0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.7 N.D. +0.5 +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 N.D. +0.6 +0.0 N.D.
growth on ricinoleic acid YNB Glucose +0.3 N.D. -0.0 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.2 +0.2 N.D.
growth on oleic acid YNB Glucose +0.6 N.D. -0.6 +0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. -0.1 +0.0 N.D.
auxotrophy Dropout Complete +0.4 N.D. -0.2 +0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 N.D. +0.3 +0.2 N.D.
growth on ypd YPD +1.8 N.D. +0.5 -0.1 N.D.
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