Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16588 and 16589 are separated by 100 nucleotides16589 and 16590 are separated by 374 nucleotides16590 and 16591 are separated by 326 nucleotides16591 and 16592 are separated by 719 nucleotides mRNA_8220: 16588 - KOG3894 SNARE protein Syntaxin 18/UFE1, at 701,037 to 702,340 16588 mRNA_8221: 16589 - KOG3140 Predicted membrane protein, at 702,441 to 703,886 16589 mRNA_8222: 16590 - KOG3140 Predicted membrane protein, at 704,261 to 706,813 16590 mRNA_8223: 16591 - K00801 FDFT1 farnesyl-diphosphate farnesyltransferase, at 707,140 to 708,844 16591 mRNA_8224: 16592 - K00948 PRPS, prsA ribose-phosphate pyrophosphokinase, at 709,564 to 711,429 16592
Group Condition mRNA_8220 mRNA_8221 mRNA_8222 mRNA_8223 mRNA_8224
16588 16589 16590 16591 16592
auxotrophy YNB Glucose N.D. -0.9 +0.1 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 -0.4 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -1.0 +0.3 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.7 +0.0 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.5 -0.1 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.6 +0.1 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 -0.3 N.D. N.D.
growth on oleic acid YNB Glucose N.D. -0.6 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.3 -0.0 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 -0.1 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.3 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 +0.0 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. -0.0 -0.1 N.D. N.D.
auxotrophy Dropout Complete N.D. -0.2 +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.0 +0.2 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.4 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 +0.2 N.D. N.D.
growth on ypd YPD N.D. +0.5 -0.1 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.5 +0.1 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.6 +0.0 N.D. N.D.
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